Naslov
Wistar-Zagreb 5HT rats : study of gene expression of 5HT synaptic elements

Autori
Bordukalo-Nikšić, Tatjana ; Čičin-Šain, Lipa ; Hranilović, Dubravka ; Jernej, Branimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 1st Croatian Congress of Neuroscience ; u: Neurologia Croatica. Supplement 52 (2003 (S) / Zurak, N. - Zagreb : Medicinski fakultet Sveučilišta u Zagrebu, 2003, 96-96

Skup
Croatian Congress of Neuroscience (1 ; 2003)

Mjesto i datum
Zagreb, Hrvatska, 21.11.2003. - 22.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
gene expression; rat brain; monoamine oxidase; semi-quantitative RT-PCR

Sažetak
By selective breeding, two sublines of rats with extremely high or low values of platelet serotonin level and platelet serotonin transporter activity have been developed (Wistar-Zagreb 5HT rats). Previous studies demonstrated significant differences between the sublines regarding the expression of platelet 5HT transporter (5HTt) at the level of both mRNA and transporter protein. Neurochemical studies indicated that similar differences might be expected in the brains of these animals. In this study, the levels of platelet and neuronal 5HTt mRNA were compared between the sublines using semi-quantitative RT-PCR. In order to find out possible adaptive/compensatory changes in brain, levels of monoamine oxidase A and B (MAO-A and B) mRNA were also compared. Studies were performed on the animals from both sublines of Wistar-Zagreb 5HT rats. Rats from the 5th, 6th and 7th breeding generation were used. Semi-quantitative RT-PCR was performed using three different housekeeping genes as references (GAPDH -actin, cyclophylin B). In accordance to the previously published results, significant difference in platelet 5HTt mRNA between the sublines was confirmed. In brain, a tendency towards higher 5HTt mRNA levels in the "high-5HT" subline was observed, as compared to the "low 5HT" subline, approaching statistical significance. Expression of MAO-A and MAO-B genes in the brains showed a slight tendency towards higher mRNA levels in the "low-5HT" subline, but without statistical significance. These results suggest that there is a possible difference in brain serotonin homeostasis between the sublines of Wistar-Zagreb 5HT rats. Determination of mRNA levels should be extended to larger sample. It is mandatory to repeat the mentioned experiments by using more sensitive method - real-time RT-PCR. However, different mechanisms of regulation of the mentioned 5HT-related genes between brain and periphery should be considered.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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