Naslov
Wistar-Zagreb 5HT rats : behavioral differences in exploratory behavior and social interaction

Autori
Hranilović, Dubravka ; Čičin-Šain, Lipa ; Bordukalo-Nikšić, Tatjana ; Jernej, Branimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 1st Croatian Congress of Neuroscience ; u: Neurologia Croatica. Supplement 52 (2003 (S) / Zurak, N. - Zagreb : Medicinski fakultet Sveučilišta u Zagrebu, 2003, 89-89

Skup
Croatian Congress of Neuroscience (1 ; 2003)

Mjesto i datum
Zagreb, Hrvatska, 21.11.2003. - 22.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
monoamine oxidase; gene polymorphism; migraine

Sažetak
By breeding selection for the extreme values of platelet 5HT level and platelet 5HT uptake, two sublines of Wistar-derived rats, with constitutionally high or low values of both parameters, have been developed (Wistar-Zagreb 5HT rats). Molecular genetic studies demonstrated that the observed differences were underlied by differences in expression of platelet 5HT transporter between the sublines at both, protein and mRNA levels. Neurochemical studies indicated that similar differences between the sublines might be expected in the expression of neuronal 5HT transporter. In order to search for the potential alterations in 5HT functioning in brains of the mentioned animals, we investigated two types of behavior known to be influenced by serotonergic activity: exploratory behavior and social interaction. Exploratory behavior was investigated as number of nose pokes on a hole board. Social interaction between the two rats was measured in open field as time spent in active contact. Results. Animals from "low 5HT" subline showed significantly higher values of both measured parameters than the animals from "high 5HT" subline: 14.9 ± ; 5.7 vs. 11.3 ± ; 4.4 nose pokes (p=0.0172, N=49 animals) and 264 ± ; 31 vs. 200 ± ; 26 seconds spent in active contact (p=0.00003, N=24 pairs). The results indicate increased exploratory behavior and reduced social interaction in animals from the "low 5HT" subline in comparison to the "high 5HT" subline. Our findings suggest that these selectively bred animals posses alterations in brain 5HT homeostasis, as demonstrated by the mentioned 5HT-related behaviors, and could be used as a model for studies of central 5HT (dis)regulation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA