Naslov
The activities of hepatic biotransformation enzymes of turkey embryos and turkey

Autori
Prevendar Crnić, Andreja ; Sakar, Darko ; Pompe-Gotal, Jelena ; Biđin, Zdenko ; Pokrić, Biserka

Izvornik
Veterinarski arhiv (0372-5480) 73 (2003), 4; 211-220

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
turkey embryo ; turkey ; biotransformation enzymes

Sažetak
The activities of two enzymes from phase I of biotransformation, aniline hydroxylase and ethylmorphine N-demethylase were investigated in turkey embryos and poults. Measurements were performed five times prior to hatching, at the 18th, 22nd, 24th, 26th and 27th day of embryos’ life and five times after hatching, at the 1st, 5th, 9th, 16th and 26th day of turkeys’ life. Ten days prior to hatching the enzyme activities in embryo livers amounted to 116.8   1.6 nmol p-aminophenol/g liver/30 min, and 222.8   25.0 nmol formaldehyde/g liver/30 min for aniline hydroxylase and ethylmorphine Ndemethylase, respectively. A three- and two-fold increase in aniline hydroxylase and ethylmorphine N-demethylase activities was observed immediately after hatching (1st day) in relation to 27-day-old embryos, respectively. Thereafter activities continued to rise and in 26-day-old poults they amounted to 672.9   10.3 nmol p-aminophenol/g liver/30 min, and 2672.6   27.9 nmol formaldehyde/g liver/30min. Body masses, as well as absolute and relative liver masses were measured after hatching. The ratio between liver/body mass showed that relative liver mass increased from the 1st to the 9th day of life, followed by a constant slight decrease until the 26th day of life. These results generated in physiological conditions indicate that biotransformation phase I reactions are higher at the end of first month of turkey life than those in turkey embryos and in newly-hatched turkey. Thereby, liver capacity for hydroxylation and N-demethylation of xenobiotics and endogenous compounds gradually increase in young turkey.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina

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