Naslov
Specific quantification of human genomes from low copy number DNA samples in forensic and ancient DNA studies.

Autori
Alonso, Antonio ; Martin, Pablo ; Albarran, Cristina ; Garcia, Pilar ; Primorac, Dragan ; Garcia, Oscar ; Fernandez de Simon, Lourdes ; Garcia-Hirschfeld, Julia ; Sancho, Manuel ; Fernandez-Piqueras, Jose

Izvornik
Croatian Medical Journal (0353-9504) 44 (2003), 3; 273-280

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA; DNA fingerprinting; DNA mitochondrial; evolution; forensic medicine; polymerase chain reaction; tandem repeat sequences

Sažetak
We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has been put on the applicability of recently described different real-time PCR designs targeting different fragments of the HV1 mtDNA control region, and a segment of the X-Y homologous amelogenin gene. The importance of these quantitative assays is to ensure the consistency of low copy number DNA typing (STR profiling and mtDNA sequencing).

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA