Naslov
Detection and characterization of measles virus strains in cases of subacute sclerosing panencephalitis in Croatia

Autori
Forčić, Dubravko ; Baričević, Marijana ; Zgorelec, Renata ; Kružić, Vladimira ; Mažuran, Renata

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
The Third European-American School in Forensic Genetic and Mayo Clinic Course in Advanced Molecular and Cellular Medicine, Final Program and Abstracts / Zlatko Kniewald - Zagreb : Akademija tehničkih znanosti Hrvatske (HATZ), 2003, 88-88

ISBN
953-7076-07-5

Skup
The Third European-American School in Forensic Genetic and Mayo Clinic Course in Advanced Molecular and Cellular Medicine

Mjesto i datum
Zagreb, Hrvatska, 01.09.2003. - 05.09.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
SSPE; measles; genotype; mutations

Sažetak
We describe here the molecular characteristics of two cases of subacute sclerosing panencephalitis (SSPE) in the children from Croatia diagnosed in 2002: a 4-year old girl and 8– year old boy who apparently, according to the medical records, both had acute measles at the age of 6 months. However, they were both vaccinated against measles at the age of 12 months. The patients were hospitalized and treated for encephalitis 3, 5 and 7 years after the onset of acute measles. Measurement of measles antibody in the serum and cerebrospinal fluid (CSF) was not helpful in the diagnosis of SSPE, as well as RT-PCR used to amplify measles genes directly from blood and CSF. In contrast, RT-PCR used to amplify measles genes directly from brain tissue proved to be useful. The minimum amount of sequence data required for genotyping a clinical specimen is 450 nucleotides that code for the COOH-terminal of the N protein, but complete N, M and H gene sequences were also obtained from the brain tissues. Sequence analysis was done by using a set of designated reference sequences (Weekly epidemiological record, 2001, 76, 241-248) representing different genotypes. Sequence analysis showed the genetic characteristics of wild-type measles virus which could be placed into genotype D6. On the other hand, vaccine virus in use in Croatia belongs to the genotype A. Furthermore, when sequences from these children were compared, we found mutations in analysed genes.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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