Pregled bibliografske jedinice broj: 134550
Cytomegalovirus Infection in Mice Caused by Bacteria Carring a Cloned MCMV Genome
Cytomegalovirus Infection in Mice Caused by Bacteria Carring a Cloned MCMV Genome // Final programme and abstract book ; 9th International Cytomegalovirus Workshop and 1st International Betaherpesvirus Workshop / Bruggeman, Cathrien ; Vink, Kees ; Claus-Hahn, Fia ; (ur.).
Maastricht, 2003. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 134550 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Cytomegalovirus Infection in Mice Caused by Bacteria Carring a Cloned MCMV Genome
Autori
Čičin-Šain, Luka ; Brune, Wolfram ; Bubić, Ivan ; Jonjić, Stipan ; Koszinowski, Ulrich ;
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Final programme and abstract book ; 9th International Cytomegalovirus Workshop and 1st International Betaherpesvirus Workshop
/ Bruggeman, Cathrien ; Vink, Kees ; Claus-Hahn, Fia ; - Maastricht, 2003
Skup
9th International Cytomegalovirus Workshop ; 1stInternational Betaherpesvirus Workshop
Mjesto i datum
Maastricht, Nizozemska, 20.05.2003. - 25.05.2003
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
cytomegalovirus; infection
Sažetak
Bacterial delivery systems are gaining increasing interest as potential vaccination vectors to deliver either proteins or nucleic acids for gene expression in the recipient. Bacterial delivery systems for gene expression usually contain small multicopy plasmids. We addressed the question of whether bacteria containing a single plasmid with a complete cytomegaloviral genome could reconstitute the viral replication process in vivo, and whether this would lead to specific immunity against the virus. We have cloned the murine cytomegalovirus (MCMV) genome as a bacterial artificial chromosome (BAC) maintained in E. coli. Here we show that the MCMV-BAC can be stably maintained in various strains of Salmonella typhimurium as well. Further, we show that both the S. typhimurium and E. coli, harboring the single copy MCMV-BAC reconstituted virus infection upon injection into interferon-gamma receptor deficient mice. This led to elevated titers of specific anti-MCMV antibodies, protetion against lethal MCMV challenge and strong expression of additional genes introduced into the viral genome. Thus, the reconstitution of infectious virus from live attentuated bacteria presents a novel concept for studying the potential of multivalent virus vaccines launched from bacterial vectors.
Izvorni jezik
Engleski
