Naslov
Chracterization of a gene trap integration within a gene expressed during embryo development

Autori
Ćurlin, Marija ; Kostović-Knežević, Ljiljana ; Gajović, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Croatian Congress on Molecular Life Sciences / - Opatija, 2002

Skup
1st Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
mouse; embryo; gene trap

Sažetak
Gene trap method is a method to identify new genes and analyze their function during mammalian embryonic development. Due to the random insertion of the gene trap vector in the genome of embryonic stem cells, any, presumably novel, gene can be tagged, and the corresponding mouse line carrying the gene trap mutation produced. The inserted vector contains a selectable marker (neoR) for mutation, a reporter gene (lacZ) that reflects endogenous gene expression and a polyA site that disables the 3´ part of the endogenous gene. The gene trap method simultaneously enables cloning of the mutated genes, determination of its expression pattern, and an analysis of the phenotype, which gives an insight in the function of the mutated gene. Using a gene trap method we produced a mouse line with an insertional mutation in a gene that was named lobel. We obtained the coding sequence of lobel cDNA produced from the mRNA isolated from mouse embryonic stem cells. The gene trap vector insertion is unique and it is located in the first part of the lobel gene. Expression pattern analysis performed by X-gal staining showed that lobel is expressed in the neural tube and in the heart of the mouse embryo, and in the brain of the adult mice. In order to get insight in the function of the gene we analyzed the phenotype of homozygous mutant mice. They showed no macroscopic malformations and they were fertile.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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