Naslov
STR and HLA analysis in paternity testing

Autori
Grubić, Zorana ; Štingl, Katarina ; Martinez, Natalija ; Palfi, Biserka ; Brkljačić-Šurkalović, Ljerka ; Brkljačić-Kerhin, Vesna ; Kaštelan, Andrija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The third European-american school in forensic genetics and Mayo Clinic course in advanced molecular and cellular medicine-Abstract Book / Primorac, D. ; Ivkošić Erceg, I. ; Ivkošić, A. ; Vuk-Pavlović S. ; Schanfield, M. - Zagreb, 2003

Skup
The third European-american school in forensic genetics and Mayo Clinic course in advanced molecular and cellular medicine

Mjesto i datum
Zagreb, Hrvatska, 01.09.2003. - 05.09.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
STR; HLA; paternity testing

Sažetak
STR loci became a valuable tool in paternity testing during the past decade. However, the mutation rate of STR loci is higher than that of conventional genetic markers and for that reason the chance of detecting mutation increases. During the 3-year period, 111 cases of paternity disputes were processed in our laboratory. The analysis was performed on 9 STR (TH01, VWA31, FES/FPS, F13A01, SE33, D1S1656, D12S391, D18S535, and D22S683) loci and one VNTR (D1S80) locus. DNA was isolated from peripheral blood leukocytes by standard salting-out method. STR loci were amplified by PCR and the products analysed by electrophoresis on a polyacrilamide gel in ALFexpress sequencer (Pharmacia Biotech). These cases were also tested for HLA class I and class II alleles by serology. Out of 111 cases, 22 were exclusions. In all cases, exclusion was confirmed on at least 5 loci. STR loci that were informative in majority of cases were SE33 (85.7%) and D12S391 (82.9%). The least informative loci were FES/FPS (55.6%) and F13A01 (57.9%). In 21 cases exclusions were also confirmed by HLA class I, while only in 17 cases HLA class II alleles excluded the alleged father. In three cases we detected a mutation of single STR locus (VWA31, 2xD12S391). In all three cases mutation was a deletion, which occurred at the paternal allele.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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