Naslov
Antimutagenic effects of glucosinolates on human cell lines

Autori
Durgo, Ksenija ; Osmak, Maja ; Garaj-Vrhovac, Verica ; Franekić Čolić, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts 1st Croatian Congress on Molecular Life Sciences / - Zagreb, 2002

Skup
1st Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Glucosinolates; Cruciferae family; glutathione transferases

Sažetak
Glucosinolates are a group of compounds widely distributed among the plants of Cruciferae family ( e.g., a broccoli, cauliflowers, cabbage). These compounds are easily hydrolized by the enzime myrozinase which becomes activated after mechanical destruction of the plant cell. The most common products of such hydrolisis are physiologically active copmounds like nitriles, thiocyanates and indoles. There are many evidence about inhibitory effect of indole compounds on different kinds of environmental mutagens. In this study, influence of the indole-3-carbinol and cauliflower extract on the cellular biomarkers and abberant evants were investigated. Human laryngeal carcinoma cells and human lympocytes were used as a test systems. The aim of investigation was to estimate of indole compounds have influence on P-glycoprotein activity, on the intracellular level of glutathione (GSH) and activity of glutathione transferases (GST). In order to evaluate possible cytogenic damage the analyses of structural chromosome aberrations in peripheral bllod lympocytes cultivated in vitro for 48 hours have been used. Indole compounds didn't inhibit the activity of P-glycoprotein in parental laryngeal carcinoma cells (HEp2) and their cisplatin resistant subline (CK2). This compounds caused significant increase of glutathione level and glutathione-S-transferase activity in HEp2 cells. On the contrary, in CK2 cells which have increased basal level of glutathione, after treatment with both drugs, the level of glutathione and glutathione-S-transferaze activity was decreased. Indole-3-carbinol and cauliflower extract didn't induced significant increase of chromosomal aberrations compared to the control.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA