Naslov
The role of NKG2D co-stimulation in MCMV control by CD8+ T lymphocytes

Autori
Hasan, Milena ; Bubić, Ivan ; Krmpotić, Astrid ; Yokoyama, Wayne ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th International Cytomegalovirus Workshop, Maastricht, Netherlands / Vink, Kees ; Bruggeman, Cathrien - Maastricht, 2003

Skup
9th International Cytomegalovirus Workshop

Mjesto i datum
Maastricht, Nizozemska, 20.05.2003. - 25.05.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
NKG2D; MCMV; m152; CD8 T lymphocytes

Sažetak
NKG2D is an activating NK cell receptor, expressed also by activated macrophages and CD8+ T lymphocytes. At the surface of CD8+ T cells NKG2D serves as a co-stimulatory receptor. We have recently shown that gp40, encoded by m152 MCMV gene, down-regulates cellular ligands for NKG2D receptor, thereby inhibiting NK cell activation and promoting virus growth in vivo (Krmpotic et al., MCMV glycoprotein gp40 confers virus resistance to CD8+ T cells and NK cells in vivo, Nat Immunol 3:529, 2002.). In this work we investigated the significance of NKG2D co-stimulation for CD8+ T cell-mediated control of MCMV infection in vivo. To this end NKG2D receptors on CD8+ T lymphocytes were blocked by an anti-NKG2D antibody and the cells were tested for their antiviral activity after adoptive transfer into MCMV infected syngeneic recipients. To assess the role of m152/gp40-mediated down-regulation of NKG2D ligands in CD8+ T cell-mediated virus control, the same protocol was performed for m152 deletion mutant and its revertant virus strain. Our preliminary results support the notion about the importance of NKG2D co-stimulation for virus control by CD8+ T cell in vivo.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA