Naslov
Polymorphism of CYP2C9 and CYP2D6 in Predicting Drug Metabolism Phenotype

Autori
Topić, Elizabeta ; Štefanović, Mario ; Čulav, Jadranka ; Samardžija, Marina ; Blazinić, Franciska ; Begonja, Antonija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the ..... ; u: Clinical Chemistry and Laboratory Medicine 40 (2002) (S) / - , 2002, S 125-S 125

Skup
XXX

Mjesto i datum
XX, XXX, 2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CYP2C9 ; CYP2D6 ; anticoagulant and antipsychotic drugs ; side effect

Sažetak
CYP2C9 and CYP2D6 are highly polymorphic enzymes metabolizing anticoagulant and antipsychotic drugs, respectively. Two CYP2C9 alleles, 2C9*2 and 2C9*3, encode for enzymes with 16%– ; ; 20% and 5% of normal activity, respectively. Six CYP2D6 alleles (*3, *4, *5, *6, *7 and *8) encode for inactive enzymes. The aim was to study frequency of 2C9 and 2D6 alleles in healthy Croatian population and assess the association between CYP2D6 polymorphism and side effect in psychiatric patients (n=87) on therapy more than 3 years. The CYP2C9 alleles were identified by PCR-RFLP and CYP2D6 alleles by multiplex PCR. In controls, the frequency of CYP2C9 *2 and *3 allele was 12.7% and 2.9%, respectively, somewhat lower than in Caucasians. Genotype distribution showed 27.5% as heterozygous with one, and 2.0% as heterozygous with two mutations. The results on 2D6 indicated a significant allele (p=0.015, odds=2.542), genotype (p=0.016) and phenotype (p=0.025, odds=5.020) difference between controls and psychiatric patients. Considerably higher frequency of heterozygous (34.2%) and homozygous (13.2%) 2D6*4 allele was found in patients with (n=39) and without (n=48) side effect. The odds ratio of 2.626 for 2D6*4 and of 5.333 for 2D6*6 alleles suggested their significant association with side effect in patients on long-term therapy.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA