Naslov
Cytotoxicity of polybrominated diphenyl ethers and polychlorinated biphenyls on human neuroblastoma (SH-SY5Y) and kidney (HEK293) cells

Autori
Klinčić, Darija ; Zandona, Antonio ; Dvoršćak, Marija ; Jagić, Karla ; Katalinić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference - Present and Future of toxicology: challenges and opportunities / - , 2023, 288-289

ISBN
978-86-917867-3-1

Skup
13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference - Present and Future of toxicology: challenges and opportunities

Mjesto i datum
Beograd, Srbija, 10.05.2023. - 12.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
polychlorinated biphenyls ; polybrominated diphenyl ethers ; human neuroblastoma (SH-SY5Y) cells ; kidney (HEK293) cells ; cytotoxicity

Sažetak
Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) belong to a group of compounds known as persistent organic pollutants (POPs) characterized by high persistence, lipophility, bioaccumulation potential, and toxicity. Although they originate from different sources, they accumulate in the same environmental compartments and in humans. In this study, human neuroblastoma (SH-SY5Y) and kidney (HEK293) cells were exposed for 24h to the following mixtures of congeners: ΣPCB (CB-28, -52, -101, -118, -138, -153, -180), and ΣPBDE (BDE-28, -47, -99, -100, -153, -154, -183) in total concentrations of 265 and 12 ng mL-1, respectively. These values were the maximum concentrations of each pollutant group detected in individual human milk samples collected in Zagreb, Croatia during the sampling campaign 2019/2020. We observed that both ΣPBDEs and ΣPCBs had a similar effect on HEK293 cell viability, while SH-SY5Y cells were more affected by ΣPBDEs. Still, the observed decrease of 12 % in viability was not considered statistically significant by the analysis used. However, when these two cell types were exposed to a combination of ΣPBDEs and ΣPCBs (265 + 12 ng mL-1), the decrease in viability was approximately 23 % and statistically significant in comparison to control. Our result indicated that, when applied together, these two pollutant classes could act synergistically and disturb cell homeostasis. This finding raises concern with regard to human exposure to mixtures of such bioaccumulative environmental xenobiotics and considering that the tested concentrations were actually detected in human milk. Further detailed research on possible adverse effects is of the utmost importance.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA