Naslov
EVALUATION OF ANTIMICROBIAL EFFICACY OF SWEEPS AGAINST ENTEROCOCCUS FAECALIS BIOFILM

Autori
Petričević Kapetanović, Gabrijela ; Perčinić, Antonio ; Budimir, Ana ; Anić, Ivica ; Bago, Ivona

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts 9th International Congress of the School of Dental Medicine University of Zagreb / - , 2023, 193-193

Skup
9th Acta stomatol Croat. 2023 ; 57(2):191-203. International Congress of the School of Dental Medicine University of Zagreb

Mjesto i datum
Dubrovnik, Hrvatska, 24.03.2023. - 25.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Enterococcus Faecalis ; bakterijski biofilm ; SWEEPS, laserski aktivirano ispiranje
(Enterococcus Faecalis ; Bacterial Biofilm ; SWEEPS ; Laser Activated Irrigation)

Sažetak
Aim: Objective of this study was to examine the efficacy of novel Er:YAG laser activated irrigation mode, Schock Wave Enhanced Emission Photoaacoustic Streaming (SWEEPS), against Enterococcus faecalis (Ef) bacterial biofilm grown on dentinal discs in comparison to ultrasonically activated irrigation (UAI) and conventional syringe-needle irrigation (SNI). Materials and Methods: Sixty (60) standardized dentinal discs (5x5 mm), made out of human extracted teeth using Isomet saw (Isomet 1000, Buehler, Illinois, USA), were polished, sterilized in plasma and used as medium for the development of two weeks old Enterococcus faecalis biofilm (ATCC 29212, American Type Culture Collection, Manassas, VA, USA). After the incubation period, the growth of the Ef biofilm was confirmed on scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The samples were randomly divided into three experimental groups (n=20/each) according to the disinfection technique used: Group 1. SWEEPS (ER:YAG laser), Group 2. UAI, Group 3. SNI. In all groups, 3% sodium hypochlorite was used. In the positive control group, no treatment was performed. Following treatment protocol, microbiological samples were collected, serialy diluted and plated on Columbia agar plates. The number of colony forming units (CFUs) was counted after 48h and transformed into exact value based on the dilution factor. Resuts: The results were statistically analysed using Mann-Whitney U test with p value set at 5%. SWEEPS and UAI groups both showed statistically significant reduction of CFUs (colony forming unit) compared to control group (p<0.001). Intergroup analysis showed that the SWEEPS was the most successful (p<0.001) in reducing Ef CFUs with 12 of 20 samples without CFUs growth. Conclusion: Although both SWEEPS and UAI were efficient in the elimination of Ef biofilm, the SWEEPS was the most efficient in complete eradication of bacteria. Croatian Science Foundation project No. 5303

Izvorni jezik
Engleski

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