Naslov
Equivocal effect of bioactive compounds from plants

Autori
Durgo, Ksenija ; Huđek Turković, Ana ; Martinić, Arijana ; Ledenko, Ivana ; Krizmanić, Tena ; Čučković, Florentina ; Komes, Draženka ; Milić, Mirta

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts, 13th Congress of the Serbian Society of Toxicology with international participation & 1st TOXSEE Regional Conference, Belgrade, Serbia / - Beograd, 2023, 76-77

ISBN
978-86-917867-3-1

Skup
13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference - Present and Future of Toxicology: Challenges and Opportunities

Mjesto i datum
Beograd, Srbija, 10.05.2023. - 12.05.2023

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
phytochemicals ; genotoxicity ; antioxidative effect ; oxidative stress ; regulatives

Sažetak
Biologically active compounds from plants are secondary metabolites and main representatives are flavonoids, terpenoids and alkaloids. They act as antioxidants, antimicrobials, attractants or repellents or are involved in numerous processes such as cell signaling. In animal cells, these compounds processes such as oxidative stress and antioxidative events. They inhibit different enzymes or are involved in modification of xenobiotic metabolism genes. Some of these compounds can cause epigenetic mod- ification and change the endocrine regulation. Even though traditional medicinal plants are used for long periods of time and are often assumed to be safe there is lots of evidence of death cases, serious and moderate to mild adverse events caused by incorrect use of medicinal plants, dietary supplements and other traditional medicinal products. Furthermore, plants that are used either as food ingredients or in traditional medicine can not only be toxic but also mutagenic or even carcinogenic. To point out the dual effects of phytochemicals, in this presentation the results of in vitro genotoxicity testing of rosemary and dandelion leaves extracts will be presented. As a biological test system human tongue epithelial carcinoma cells CAL 27 as a repre- sentative of oral cavity were used. Examined concentrations of both extracts were relevant to those present in food or beverage and were tested on their genotoxicity and H2O2 – induced oxidative damage protection. The results have revealed that both, dandelion and rosemary extracts increased comet tail intensity. Both extracts increased micronucleus formation even at concentrations several times lower than daily recommended doses. Higher concentrations induced cell apoptosis and necrosis. On the other hand, rosemary extract showed a protective effect against hydrogen peroxide – induced oxidative damage by decreasing the number of apoptotic cells. In order to regulate and promote rational and therapeutically sound usage, medicinal plants should be integrated as policy into national healthcare systems, and guidelines on their safety, efficacy, and quality should be conducted.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Interdisciplinarne biotehničke znanosti

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