Naslov
Effects of intermittency on microbial communities, biogeochemical and ecological process in IRES ecosystem

Autori
Petrić, Ines ; Kolda, Anamarija ; Kulaš, Antonija ; Pole, Lucia ; Senko, Helena ; Gligora Udovič, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Symposium “Power of Microbes in Industry and Environment 2023”

Mjesto i datum
Poreč, Hrvatska, 15.05.2023. - 18.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
IRES ; metabarcoding ; 16S rRNA, 18S rRNA ; qPCR, microbial mat

Sažetak
Intermittent rivers and ephemeral streams (IRES) represent ubiquitous river ecosystems (51-60% of rivers worldwide) that periodically cease to flow and/or dry out. They are usually affected by anthropogenic interference aided by climate change, that has additional effect in prolonging duration of dry periods of IRES. Furthermore, many once- perennial rivers are experiencing intermittence. Microorganisms are often overlooked in IRES oriented research, although they are responsible for the stability and sustainability of riverine ecosystems. This research is aiming to contribute data on changes in the structure and functions of microbial communities as a consequence of variations in hydrological phases, especially dry periods. Krčić River (Croatia), a naturally occurring IRES in the Dinaric karst region, was chosen as “natural laboratory” to predict behavior of future river ecosystem affected by flow intermittence. Sampling of microbial mats was conducted from April 2019 to May 2020 (except March and April). Measuring of physico-chemical parameters and nutrients was performed, as well as extraction of the total DNA and RNA for 16S rRNA and 18S rRNA gene amplicon sequencing, while selected functional genes (nifH, amoA, narG, nirS, nirK, nosZ, rbcl, anammox 16S rRNA, sox and dsrB) were determined by the qPCR. Bioinformatic data processing and downstream analysis was performed. Alphaproteobacteria (48%) and Oxyphotobacteria (35%) dominated community of the active bacteria (averaged for the whole year and all samples). Cyanobacteria were most active in the microbial mat in May, June, October 2019, February and May 2020 (50, 4%). Alphaproteobacteria was dominating active community in April, June, July, August, November 2019 and January 2020 (67, 7%). Ochrophyta were the most active members of eukaryote community (73-92%). Sample from dry period (October) had distinct community comprised mainly of Chlorophyta (44%), Ascomycota and Basidiomycota (25%), Alveolata (12%), with high percentage of rare taxa. Out of the 12 functional genes targeted via qPCR, presence of 9 different genes participating in N cycle (nitrification/ oxidation of ammonia, denitrification, nitrogen fixation), carbon cycle (Calvin-Benson-Basham cycle, photosynthesis) and S cycle (sulfur oxidation) were detected. In conclusion, microbial mat communities should be considered as indicators, in which adaptation and evolution processes related to climate change could be studied.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

Napomena
Book of Abstract not available at the time

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