Naslov
Korištenje microgAMBI indeksa za brzu procjenu ekološkog stanja u uvjetima akvakulture
(Use of microgAMBI index for fast ecological assessment of aquaculture environment)

Autori
Kolda, Anamarija ; Gavrilović, Ana ; Jug-Dujaković, Jurica ; Borja, Angel ; Ljubešić, Zrinka ; El- Matbouli, Mansour ; Lillehaug, Atle ; Lončarević, Semir ; Perić, Lorena ; Pikelj, Kristina ; Hengl, Brigita ; Knežević, Dražen ; Vukić Lušić, Darija ; Kapetanović, Damir

Kolaboracija
HRZZ collaboration

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14th International Aquaculture Conference Book of Abstracts / - , 2023, 86-89

Skup
14th International Aquaculture Conference

Mjesto i datum
Vukovar, Hrvatska, 29.03.2023. - 31.03.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
metabarcoding ; microgAMBI ; ecological status ; microbial ecology ; aquaculture

Sažetak
Aquaculture industry is currently implementing Blue Transformation strategy to enhance sustainability of the growing production. Microbes have tremendous impact on sustainability and health in aquaculture industry. Consequently, there is a global effort for implementation of eDNA metabarcoding of microbes for routine monitoring of various environments. To assess the ecological status (ES), according to microbial assemblage composition, microgAMBI (microbial genomic-based AZTI’s Marine Biotic Index) was created. Sampling of seawater and sediment was conducted in 2018, at a small-scale seabass farm in Mali Ston Bay, Croatia near island Maslinovac and a respective control site (near island Pučenjak), in March, June, September and November. Total DNA was extracted and 16S rRNA gene (V1-V3 region) was sequenced using high-throughput sequencing technology (Illumina, UK). Based on the obtained taxonomic list, microgAMBI calculation was performed and ES of the sampling sites was assigned. Most of the samples achieved “moderate” ES, with summer farm seawater sample having “bad” ES and winter control sediment sample having “good” status. Sediment in the farm was of “poor” ecological status in warmer seasons, as did the control sediment in the summer. Overall, microgAMBI demonstrated potential in effective assessment of aquaculture and surrounding environment, providing useful information for aquaculture managers and policy makers.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA