Naslov
Biocompatibility of lipophilic deep eutectic solvents on the astrocytoma cell line 1321N1 in vitro

Autori
Jurič, Andreja ; Češi Martin ; Zandona Antonio ; Obranović, Marko ; Katalinić, Maja ; Kopjar, Nevenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference - Present and Future of Toxicology: Challenges and Opportunities / - , 2023, 286-286

Skup
13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference - Present and Future of Toxicology: Challenges and Opportunities

Mjesto i datum
Beograd, Srbija, 10.05.2023. - 12.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
deep eutectic solvents, biocompatibility, primary DNA damage

Sažetak
The use of deep eutectic solvents (DES) represents an attractive approach in the extraction of cannabidiol (CBD) from hemp. It may replace other extraction media, which could exert potential cytotoxic effects. Effective and safe extraction procedures become ever more important considering the wide application of CBD oil as an ingredient of various pharmaceutics and nutraceutics. The aim of this study was to investigate the biocompatibility of a newly developed lipophilic DES on the human astrocytoma cell line 1321N1. After 24 h of in vitro exposure to different volumes of the solvent, we evaluated cell viability using the MTS assay, while the level of primary DNA damage was measured using the alkaline comet assay. The obtained results showed no statistically significant difference in cell viability compared to the control in all of the samples. Also, exposure to DES did not provoke statistically significant changes in the value of %DNA in the comet tail (%DNA <0.03) compared to the control in all of the tested samples. Our findings demonstrated an acceptable biocompatibility of the tested DES, both on cell viability and on the genome stability of 1321N1 cells. This suggested significant potential of DES for further research and its possible application as a solvent for the extraction of CBD as well different compounds that can be used in the food industry, pharmacy and cosmetics.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Biotehnologija

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