Naslov
Maternal MT2A gene polymorphisms –5A/G, –209A/G and +838G/C influence trace element levels in healthy postpartum women and their offspring

Autori
Pašalić, Daria ; Sekovanić, Ankica ; Orct, Tatjana ; Kljaković-Gašpić, Zorana ; Lazarus, Maja ; Sulimanec Grgec, Antonija ; Stasenko, Sandra ; Matek Sarić, Marijama ; Mioč, Tatjana ; Škrgatić, Lana ; Miškulin, Iva ; Branović Čakanić, Karmen ; Piasek, Martina ; Jurasović, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / Plebani, Mario - Berlin • Boston : Walter de Gruyter, 2023, S1577-S1577

Skup
WorldLab-EuroMedLab Rome 2023

Mjesto i datum
Rim, Italija, 21.05.2023. - 25.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MT2A gene polymorphisms ; trace elements ; postpartum woman ; offspring's

Sažetak
BACKGROUND-AIM Metallothioneins (MT) are cysteine-rich multifunctional metal-binding proteins. In humans, the MT2A gene comprises24 SNPs. Three of them, MT2A −5A/G, −209A/G and +838G/C were found to be possibly associated with tissue concentrations of trace elements in the body. We evaluated the association of these maternal MT2A polymorphisms and the levels of selected essential and toxic trace elements in maternal and cord blood and the placenta. METHODS The study included 424 postpartum women who gave birth at term in the maternity ward of the hospitals in Zagreb and Zadar, Croatia. DNA was isolated from blood using the QIAamp Blood Mini Kits from Qiagen. MT2A gene polymorphisms were determined by PCR-RFLP methods using BsgI (– 5A/G) and SmaI (–209A/G) restriction enzymes(New England BioLabs) or by qPCR (+838G/C) using Taqman assay (Applied Biosystems). Elements were analyzed by ICP-MS. Multiple linear regression analysis was used to evaluate the relationship between MT2A gene polymorphisms and trace elements in maternal blood, placenta and cord blood together with the factors contributing to trace element concentrations (maternal smoking and fish consumption) and other potential confounding factors (age, education, pregnancy weight gain and parity). RESULTS We found negative associations between MT2A – 209A/G SNP and Pb in maternal blood as well as with Hg in maternal and cord blood and the placenta. MT2A +838G/C was negatively associated with Pb in cord blood and positively with As in maternal and cord blood. Negative associations were also found between combinations of MT2A –5A/G and –209A/G polymorphisms and As in cord blood, MT2A –5A/G and +838G/C and Cu in cord blood as well as between a combination of all three studied MT2A SNPs and Fe in the placenta. CONCLUSIONS This study provides new evidence on the association between MT2A gene polymorphisms and trace element levels invulnerable population groups of mother-infant pairs. The studied MT2A gene polymorphisms, single or combined, were negatively associated with several trace elements in maternal, placental and fetal compartments, with the exception of the MT2A +838G/C SNP and As positive association.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA