Naslov
Exploring the Interplay between AQP3, NRF2, Oxidative Stress, and Breast Cancer Progression

Autori
Mlinarić, Monika ; Milković, Lidija ; Čipak Gašparović, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
REDOX Biology Congress, Free Radical Biology and Medicine, Volume 201, Supplement 1 / Davies, Kelvin J. A. - : ScienceDirect, 2023

Skup
Annual Meeting of the Society for Free Radical Research - Europe 2023

Mjesto i datum
Beč, Austrija, 06.06.2023. - 09.06.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
NRF2 ; AQP3 ; breast cancer ; oxidative stress

Sažetak
Oxidative stress (OS) is described as an imbalance between the production of reactive oxygen species (ROS) and the ability to neutralize them, and it has been linked to the development and progression of cancer. The antioxidative system is in charge of maintaining redox balance, but are there more players involved? Aquaporins (AQP) are transmembrane proteins involved in the regulation of water transport across cellular membranes, but besides water, they can transport other small molecules, such as glycerol and urea, and interestingly hydrogen peroxide (H2O2). By facilitating the H2O2 transport, AQPs could have a role in OS regulation, and some studies have suggested their protective effects, while others have described them as promoting cellular proliferation and invasiveness. Our research is focused on AQP3, involved in the regulation of cell growth and migration, as well as cancer development. Its expression was found to be elevated and associated with poor prognosis and increased metastasis in breast cancer patients, so understanding the role of AQP3 in OS regulation may provide insight into new strategies for breast cancer prevention and treatment. On three breast cancer cell lines, hormone positive, HER2 positive and triple negative, and a non-tumorigenic cell line, we investigated the effect of a two-week exposure to physiological levels of OS on the expression of AQP3 and the antioxidant regulator NRF2, and its regulators Keap1 and GSK-3β. We also assessed cell viability and proliferation, as well as migration ability. The effect of OS is cell- type specific. In cancer cell lines, but not in the non-tumorigenic cell line, we observed an upregulation of AQP3 expression, accompanied by increased migration ability or increased cell viability. These results suggest AQP3 may be a part of the cellular response to OS, but more research is needed to fully understand its role.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA