Naslov
TNF-α, IL-1α and IL-10 - potential inflammatory biomarkers in Alzheimer´s disease

Autori
Vuic, Barbara ; Culjak, Marija ; Milos, Tina ; Nikolac Perkovic, Matea ; Uzun, Suzana ; Nedic Erjavec, Gordana ; Tudor, Lucija ; Konjevod, Marcela ; Kozumplik, Oliver ; Mimica, Ninoslav ; Pivac, Nela ; Svob Strac, Dubravka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / - , 2022, 53-54

Skup
Inflammation and Proteinopathy in ALS/FTD Spectrum Disorder

Mjesto i datum
Rijeka, Hrvatska, 30.06.2022. - 03.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
TNF-α, IL-1α, IL-10, Alzheimer´s disease, MMSE

Sažetak
Mild cognitive impairment (MCI) is defined by memory deficits that do not significantly impact daily functioning. However, around 10-15% of subjects with MCI develop Alzheimer´s disease (AD) each year and 80% of MCI subjects will be subsequently diagnosed with AD. AD is the most common cause of dementia characterized by the accumulation of senile plaques and neurofibrillary tangles in the brain, which result in neurodegeneration and progressive deterioration of cognitive functions. At present, clinical diagnosis of (probable) AD is established through a combination of clinical symptoms, cognitive screening tests, detailed neuropsychological testing and imaging techniques. Moreover, so far, there are only symptomatic treatments that are trying to counterbalance the neurotransmitter disturbance in AD. Therefore, a wide range of multidisciplinary research has focused on the AD pathophysiology, specific biomarkers, and new therapeutic options for its prevention and treatment. More recently, the presence of a sustained immune response in the brain has emerged as a significant factor underlying the AD pathology. The complex interaction between pro- inflammatory (TNF-α, IL-1α) and anti-inflammatory (IL-10) cytokines, and their imbalance could be the critical risk factor for AD development. The aim of this study was to investigate possible associations of TNF-α (rs1800629), IL-1-α (rs1800587) and IL-10 (rs1800896) gene polymorphisms with AD, as well as to determine serum TNF-α, IL-1α and IL-10 levels in AD patients and subjects with MCI. Study enrolled 74 patients with AD and 96 subjects with MCI. Genomic DNA was extracted from peripheral blood by a salting out method. Genotyping was performed using Real-time PCR. The concentrations of TNF-α, IL-1α and IL-10 cytokines were determined by ELISA. Data was evaluated using GraphPad Prism version 4.00. No significant associations of TNF-α, IL-1α and IL-10 polymorphisms with AD were observed. However, patients with AD had significantly lower IL-1α and IL-10, as well as significantly higher TNF-α serum levels compared to subjects with MCI. Dementia severity (MMSE scores) was positively (IL-1α and IL-10) or negatively (TNF-α) correlated with the serum levels of investigated cytokines. The study confirmed the important role of the immune system in AD, suggesting dysregulation in the pro- and anti-inflammatory response. Further research is needed to clarify the potential of IL-1α, TNF-α and IL-10 as peripheral biomarkers in AD.

Izvorni jezik
Engleski

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