Naslov
Comparison of platelets in additive solution pooled and stored in three different pooling system

Autori
Liker, Milica ; Strauss Patko, Maja, Hećimović, Ana ; Vuk, Tomislav ; Gojčeta, Koraljka ; Jukić, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Vox Sanguis, International Journal of Blood Transfuion Medicine / - , 2017, 137-138

Skup
27th Regional Congress of the ISBT

Mjesto i datum
Kopenhagen, Danska, 17.06.2017. - 21.06.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sustavi za puliranje, koncentrat trombocita
(pooling system, platelet concentrate)

Sažetak
The in vitro quality of platelet concentrates (PCs) and the in vivo effectiveness of platelets (PLTs) depend on various factors like leukoreduction, storage temperature, gas exchange capabilities of the PLT storage container, PLT concentration in the storage container and duration of storage. Significant number of these factors depend on the characteristics of pooling systems components such as leukoreduction filters and PLT storage containers. AIMS Evaluation in vitro quality of BC-derived platelets in additive solution prepared and stored in three different pooling system for 5 days: Haemonetics (ATSBC 1EPSB) and Fresenius (PT52600) with autostop filters and Therumo (TF*FP0610M1) with non-autostop filter. METHODS Donor differences were avoided by applying pool- and-split design. Twelve ABO and RhD-matched BCs were pooled, mixed and then equally divided into the three pooling containers of different PLT pooling systems, each of which was supplemented with one unit of SSP+ additive solution (250 mL). After soft-spin centrifugation PLTrich supernatant was pressed using a blood component separator (Optipress II, Fenwal). First phase of separation (until RBCs reach top optic sensor) was done automatically and second phase manually. Manual pressing was stopped at the moment when the filter was completely filled with RBCs (in case of autostop filters) or at the moment when the RBCs left the outlet of the filter (in case of non- autostop filter). Ten triplicates of PCs were prepared and stored in Haemonetics, Fresenius and Terumo system for 5 days. On days 1 and 5 of storage, samples were tested for platelet concentration, mean platelet volume (MPV), pO2, pCO2, glucose, lactate, bicarbonate, pH, CD62P. RESULTS All platelet concentrates met national and Council of Europe guidelines for platelet products. There were no statistically significant differences between Haemonetics, Fresenius and Terumo systems regards the number of residual leucocytes, MPV, bicarbonate, pO2 and CD62P. PCs in Terumo system showed highest PLT recovery of 79, 5% from BC pool compared to Haemonetics 74, 7% and Fresenius 73, 4% (p<0.05). PCs in Terumo on Day 5 had highest PLT count and concentration (p<0.05). PCs in Terumo had lowest pH (p<0.05) but still well above the limit (>6, 4) on Day 5. pCO2 decreased during storage in all pooling systems but PCs in Fresenius had lowest pCO2 on Day 5 (p<0.05). Glucose decreased and lactate increased during storage for PCs in all pooling systems. PCs in Terumo system had higher glucose metabolism (p<0.05) than PCs in Fresenius and Haemonetics system as seen by a larger reduction in the glucose concentration and higher formation of lactate than PCs in Haemonetics and Fresenius system. CONCLUSION Although PCs begun as identical BC pools, they were processed using different pooling systems to evaluate effect of different leucoreduction filters and storage containers on quality of PCs. PCs in Terumo system had highest recovery and platelet concentration at the end of storage, although the pH and glucose level were lower than for other two pooling systems which suggest higher PLT metabolism. This may be result of higher number of PLT in PCs. However, pH level was within acceptable range during 5 days storage period for PCs in Terumo.

Izvorni jezik
Engleski

POVEZANOST RADA