Naslov
Widespread kallikrein expression during human kidney development becomes restricted to tubules with a reduced number of primary cilia in postnatal kidneys

Autori
Ogorevc, Marin ; Gotovac, Ivona ; Mardesic, Snjezana ; Vukojevic, Katarina ; Saraga, Marijan ; Glavina Durdov, Merica ; Saraga-Babic, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
5th International Renal Pathology Conference

Mjesto i datum
Zagreb, Hrvatska, 18.05.2023. - 20.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Primary cilia ; Kallikrein ; Kidney Development

Sažetak
Aims: The primary cilium is a signalling organelle present in most cell types and it has a crucial role in the regulation of development and homeostasis. Kallikrein is an enzyme that is part of the kallilkrein-kinin system involved in regulation of natriuresis and blood pressure. Our goal was to determine the relationship between primary cilia and kallikrein expression in the developing human mesonephros and metanephros and postnatal kidneys. Methods: We used double immunofluorescence to analyze kallikrein and acetylated α-tubulin (primary cilia marker) expression in 5- to 16- week-old human conceptuses and postnatal kidneys. The density of primary cilia was calculated using ImageJ as the ratio of the number of cilia counted and the length of the apical surface of tubules in mm. Statistical analysis was performed in GraphPad Prism. Results: Our preliminary results show that, during development, diffuse kallikrein expression characterized both podocytes and parietal epithelial cells (PECs) of renal corpuscles, while only PECs retained primary cilia past the embryonic period (Figure 1, a-c). In the postnatal kidney, the entire corpuscle became devoid of kallikrein, while only some tubules, morphologically resembling proximal tubules, demonstrated strong apical expression (Figure 1, d). When comparing postnatal kidney tubules with and without kallikrein expression, a noticeable reduction in the number of primary cilia in kallikrein-positive tubules was observed (Figure 1, e-j). Statistical analysis showed a significant difference (p<0.0001) in the average density of primary cilia between kallikrein-positive (4.2 cilia/mm) and kallikrein-negative (21 cilia/mm) tubules. Conclusion: Extensive kallikrein expression present during nephrogenesis becomes limited to certain tubules in the mature kidney and those tubules have comparatively less primary cilia than the rest. This implies an inverse relationship between kallikrein expression and primary cilium signalling.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA