Naslov
Synergy of NMR and FTIR spectroscopy in the characterization of asymmetric liposomes

Autori
Bakarić, Danijela ; Pašalić, Lea ; Maleš, Petra ; Pem, Barbara ; Čikoš, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th Adriatic NMR Conference : Book of Abstracts / Bregović, Nikola ; Namjesnik, Danijel ; Novak, Predrag ; Parlov Vuković, Jelena - Zagreb : Hrvatsko kemijsko društvo, 2023, 41-41

Skup
7th Adriatic NMR 2023

Mjesto i datum
Mali Ston, Hrvatska, 01.06.2023. - 04.06.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Asymmetric liposomes ; NMR spectroscopy ; DSC ; FTIR spectroscopy

Sažetak
Although asymmetric liposomes are nowadays considered the most suitable representatives of plasma membranes, their rather challenging preparation accompanied by the use of limiting combinations of lipids, is further complicated by their far from straightforward characterization. [1] One of the indispensable techniques in confirming the asymmetric distribution of exclusively phosphatidylcholine (PC) lipids is 1H NMR spectroscopy, which, in the presence of lanthanide ions such as Pr3+, can distinguish the protons of choline group (−N(CH3)3+) located in the inner or outer membrane leaflet (Figure 1).[2] With the aim of identifying PC lipid molecules in a certain leaflet of asymmetric liposomes, we prepared them from 1, 2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC) and 1, 2-dipalmitoyl-sn- glycero-3-phosphoserine (DPPS), while the response of −N(CH3)3+ group was characterized with 1H NMR and for the first time by FTIR spectroscopy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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