Pregled bibliografske jedinice broj: 1278201
A simple rodent subcutaneous assay for identification of new osteoinductive molecules: The key method for screening of novel bone regeneration implants
A simple rodent subcutaneous assay for identification of new osteoinductive molecules: The key method for screening of novel bone regeneration implants // Rad Hrvatske akademije znanosti i umjetnosti. Medicinske znanosti, 60-61 (2022), 40-53 doi:10.21857/y54jofvgxm (podatak o recenziji nije dostupan, pregledni rad, znanstveni)
CROSBI ID: 1278201 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A simple rodent subcutaneous assay for
identification of new osteoinductive molecules:
The key method for screening of novel bone
regeneration implants
Autori
Štoković, Nikola ; Ivanjko, Natalia ; Milešević, Marina ; Kuber Sampath, T. ; Vukičević, Slobodan
Izvornik
Rad Hrvatske akademije znanosti i umjetnosti. Medicinske znanosti (1330-5301) 60-61
(2022);
40-53
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni
Ključne riječi
Bone regeneration ; bone regeneration implants
Sažetak
Treatment of large bone defects and degenerative diseases of the spine is among the most challenging and still unresolved issues in clinical medicine. Therefore, substantial effort has been devoted to the development of novel bone regenerative therapies. Due to their potent osteoinductive properties, Bone Morphogenetic Proteins (BMPs) have been the basis for the development of novel strategies for bone regeneration. The use of animal models is an indispensable part of the preclinical testing of novel therapeutic solutions. The rat subcutaneous assay became the initial screening procedure for the evaluation of promising BMP-based osteoinductive devices for bone regeneration because only osteogenic BMPs can induce new bone at any ectopic rodent site. Moreover, this model is used for research on the mechanisms of ectopic bone formation as well as for the evaluation of the inflammatory response to different materials. In this review, we provided an overview of the assay development and previously conducted studies with different methods (flow cytometry, histological and microCT analyses) for the study outcome evaluation. Moreover, we addressed essential issues in the experimental design such as the follow-up period and the sample size. The rat subcutaneous bone induction assay layed the foundation for isolation and identification of BMPs followed by testing of new osteogenic devices in higher animal species and humans
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
--KK.01.1.1.01.0008 - Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platormi i potencijala (CERRM) (Ježek, Davor; Vukičević, Slobodan) ( CroRIS)
EK-FP7-279239 - Novel Bone Morphogenetic Protein-6 Biocompatible Carrier Device for Bone Regeneration (OSTEOGROW) (Vikić-Topić, Smiljka; Đurđević, Dragan, EK - FP7-HEALTH-2011-two-stage) ( CroRIS)
--779340 - Novi lijek za regeneraciju kostiju Osteogrow: terapeutsko rješenje za lumbalne bolove u leđima (OSTEOproSPINE) (Vukičević, Slobodan) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Slobodan Vukičević
(autor)
Nikola Štoković
(autor)
Natalia Ivanjko
(autor)
Marina Milešević
(autor)