Long-term posterolateral spinal fusion in rabbits induced by rhBMP6 applied in autologous blood coagulum with synthetic ceramics

Stokovic, Nikola; Ivanjko, Natalia; Pecin, Marko; Erjavec, Igor; Smajlović, Ana; Milesevic, Marina; Karlovic, Sven; Capak, Hrvoje; Vrbanac, Zoran; Maticic, Drazen; Vukicevic, Slobodan

doi:10.1038/s41598-022-14931-2

Pregled bibliografske jedinice broj: 1278190

Long-term posterolateral spinal fusion in rabbits induced by rhBMP6 applied in autologous blood coagulum with synthetic ceramics

Stokovic, Nikola; Ivanjko, Natalia; Pecin, Marko; Erjavec, Igor; Smajlović, Ana; Milesevic, Marina; Karlovic, Sven; Capak, Hrvoje; Vrbanac, Zoran; Maticic, Drazen; Vukicevic, Slobodan

Long-term posterolateral spinal fusion in rabbits induced by rhBMP6 applied in autologous blood coagulum with synthetic ceramics // Scientific Reports, 12 (2022), 1; 11649, 16 doi:10.1038/s41598-022-14931-2 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1278190 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Long-term posterolateral spinal fusion in rabbits induced by rhBMP6 applied in autologous blood coagulum with synthetic ceramics

Autori
Stokovic, Nikola ; Ivanjko, Natalia ; Pecin, Marko ; Erjavec, Igor ; Smajlović, Ana ; Milesevic, Marina ; Karlovic, Sven ; Capak, Hrvoje ; Vrbanac, Zoran ; Maticic, Drazen ; Vukicevic, Slobodan

Izvornik
Scientific Reports (2045-2322) 12 (2022), 1; 11649, 16

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
BMP ; Bone morphogenetic proteins ; bone regeneration ; spinal fusion

Sažetak
Autologous bone graft substitute (ABGS) containing rhBMP6 in autologous blood coagulum (Osteogrow) is a novel therapeutic solution for bone regeneration. This study is aimed to investigate the long-term outcome of ABGS with synthetic ceramics (Osteogrow-C) in rabbit posterolateral spinal fusion (PLF) model. Osteogrow-C implants were implanted bilaterally between rabbit lumbar transverse processes. We compared the outcome following implantation of ABGS with ceramic particles of different chemical composition (TCP and biphasic ceramics containing both TCP and HA) and size (500–1700 µm and 74–420 µm). Outcome was analyzed after 14 and 27 weeks by microCT, histology, and biomechanical analyses. Successful bilateral spinal fusion was observed in all animals at the end of observation period. Chemical composition of ceramic particles has impact on the PLF outcome via resorption of TCP ceramics, while ceramics containing HA were only partially resorbed. Moreover, persistence of ceramic particles subsequently resulted with an increased bone volume in implants with small particles containing high proportion of HA. ABGS (rhBMP6/ABC) with various synthetic ceramic particles promoted spinal fusion in rabbits. This is the first presentation of BMP-mediated ectopic bone formation in rabbit PLF model with radiological, histological, and biomechanical features over a time course of up to 27 weeks.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA

Projekti:
--KK.01.1.1.01.0008 - Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platormi i potencijala (CERRM) (Ježek, Davor; Vukičević, Slobodan) ( CroRIS)
--779340 - Novi lijek za regeneraciju kostiju Osteogrow: terapeutsko rješenje za lumbalne bolove u leđima (OSTEOproSPINE) (Vukičević, Slobodan) ( CroRIS)
EK-FP7-279239 - Novel Bone Morphogenetic Protein-6 Biocompatible Carrier Device for Bone Regeneration (OSTEOGROW) (Vikić-Topić, Smiljka; Đurđević, Dragan, EK - FP7-HEALTH-2011-two-stage) ( CroRIS)

Ustanove:
Veterinarski fakultet, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb,
Medicinski fakultet, Zagreb

Profili:

Nikola Štoković (autor)