Naslov
Glucose, Insulin and Oxygen Modulate Expression of Serotonin-Regulating Genes in Human First- Trimester Trophoblast Cell Line ACH-3P

Autori
Perić, Maja ; Horvatiček, Marina ; Tandl, Veronika ; Bečeheli, Ivona ; Majali-Martinez, Alejandro ; Desoye, Gernot ; Štefulj, Jasminka

Izvornik
Biomedicines (2227-9059) 11 (2023), 6; 1619, 15

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
gestational diabetes mellitus ; obesity ; 5-HT ; 5-HTT ; placenta ; human trophoblasts ; early pregnancy ; hyperglycemia ; hyperinsulinemia ; hypoxia

Sažetak
Serotonin signaling plays an important role in regulating development and functions of the placenta. We hypothesized that metabolic disturbances associated with maternal obesity and/or gestational diabetes mellitus (GDM) affect placental serotonin homeostasis. Therefore, we examined the effects of high glucose (25 mM) and insulin (10 nM)—two hallmarks of maternal obesity and GDM—on mRNA expression of key regulators of serotonin homeostasis, including serotonin transporter (SERT), tryptophan hydroxylase 1 (TPH1), and monoamine oxidase A (MAOA), in the first-trimester trophoblast cell line ACH-3P, focusing on oxygen levels characteristic of early human placental development. Glucose downregulated expression of SERT and MAOA independently of oxygen level and upregulated expression of TPH1 at 6.5% oxygen but not at 2.5% oxygen. Compared to 6.5% oxygen, 2.5% oxygen upregulated SERT and downregulated TPH1 expression, with no effect on MAOA expression. Insulin upregulated SERT only at 2.5% oxygen but had no effect on TPH1 and MAOA expression. These results suggest that maternal metabolic alterations in early pregnancy may be a driving force for changes in placental serotonin homeostasis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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