Naslov
NMR and fluorometric studies of bioactive macrozone interactions with their biological targets

Autori
Jednačak, Tomislav ; Mikulandra, Ivana ; Smokrović, Kristina ; Kapustić, Monika ; Delaš, Kristina ; Piantanida, Ivo ; Jurković, Marta ; Zangger, Klaus ; Novak, Predrag

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Adriatic NMR 2023 - Book of Abstracts / Bregović, Nikola ; Namjesnik, Danijel ; Novak, Predrag ; Parlov Vuković, Jelena - Zagreb : Hrvatsko kemijsko društvo, 2023, 50-50

Skup
Adriatic NMR Conference 2023

Mjesto i datum
Mali Ston, Hrvatska, 01.06.2023. - 04.06.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
macrozones, STD, WaterLOGSY, fluorometry

Sažetak
Macrozones are new conjugates of azithromycin and thiosemicarbazones, which exhibit very good in vitro antibacterial activities against susceptible and some resistant bacterial strains thus showing a potential for further development. In this work, we applied a combination of NMR and fluorometric methods to study interactions of bioactive macrozones with their biological targets: bovine serum albumin and ribosome isolated from Escherichia coli. Saturation transfer difference (STD) and water-ligand observed via gradient spectroscopy (WaterLOGSY) spectra provided valuable data on ligand orientation, solvent exposure, binding epitopes and specificity. The binding stoichiometry and association constants were calculated from fluorometric data. These results can serve as a good starting point for the design of new macrozones and their metal complexes with improved bioactivity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA