Naslov
Strict Regulation of the KEAP1-NRF2 Pathway and its Interaction with the Aquaporin 3 Expression in Breast Cancer Cell Lines

Autori
Mlinarić, Monika ; Čipak Gašparović, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
“HDIR-6: Targeting Cancer” The 6th Meeting of the Croatian Association for Cancer Research with International Participation : Book of Abstracts / Ozretić, Petar - Zagreb : Hrvatsko društvo za istraživanje raka (HDIR), 2022, 40-40

ISBN
978-953-48672-1-1

Skup
“HDIR-6: Targeting Cancer” The 6th Meeting of the Croatian Association for Cancer Research with International Participation

Mjesto i datum
Zagreb, Hrvatska, 10.11.2022. - 12.11.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
AQP3 ; NRF2 ; Keap1 ; breast cancer ; oxidative stress

Sažetak
Aquaporins (AQP) are membrane proteins that facilitate the transport of water and other small uncharged solutes across the plasma membrane, which makes them important in the regulation of cellular water homeostasis, proliferation, and migration. Several AQPs can transport hydrogen peroxide and are therefore named peroxiporins, who by facilitating hydrogen peroxide in and potentially out of the cell influence cell’s oxidative status. In the state of oxidative stress, characterized by an imbalance between oxidants and antioxidants, AQPs have a significant role and could contribute to oxidative damage in the cell. To prevent oxidative damage and associated undesirable effects, cells have a strong antioxidant system. As a major regulator of cellular resistance to oxidative stress, Nrf2 (nuclear factor erythroid 2–related factor 2) induces the expression of many antioxidant genes and therefore regulates cellular response to oxidative stress. Since it has an important role in fine-tuning of ROS, Nrf2 regulation is crucial. Keap1 (Kelch Like ECH Associated Protein 1) acts as a sensor for oxidative stress and is responsible for Nrf2 ubiquitination and degradation in physiological conditions, and its activation induced by oxidants. Once activated Nrf2 translocates to the nucleus where it exerts its effect. The Nrf2-Keap1 axis, as well as AQPs, are often dysregulated in tumors, who use them for their protection against constantly present oxidative stress. Our aim was to study the Nrf2 regulation in breast cancer cell lines, and to see does it somehow affect the AQP expression. According to the literature, AQP3 is often overexpressed in breast cancer cells compared to normal breast cells, so we put our efforts into finding its potential interaction with Nrf2. Our results indicate a strong regulation of Nrf2 activation independent of its expression. Cell line-dependent changes in AQP expression were also observed, but additional research is needed to determine whether there is an interaction between these proteins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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