Naslov
Effect of lipid raft disruption on AQP3, AQP5, and EFGR pathway

Autori
Mlinarić, Monika ; Milković, Lidija ; Čipak Gašparović, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Translational Potential of Cancer Research in Serbia : abstract book / Đorđić Crnogorac, Marija ; Nedeljković, Milica - Beograd : Srpsko društvo istraživača raka, 2021, 55-55

ISBN
978-86-919183-3-0

Skup
5th Congress of the Serbian Association for Cancer Research with International Participation SDIR-5 “Translational Potential of Cancer Research in Serbia“

Mjesto i datum
Online ; Beograd, Srbija, 03.12.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aquaporin ; breast cancer ; EGFR ; lipid raft disruption

Sažetak
Background: Breast cancer is the leading cause of morbidity and mortality of cancer in women. Therefore, there is a need for understanding modifications in biological processes and signaling pathways that contribute to breast cancer malignancy. Oxidative stress is certainly an important factor that encourages tumor growth by supporting several hallmarks of cancer. Peroxiporins are aquaporins that channel hydrogen peroxide and could, therefore, play a role in regulating intracellular levels of oxidative stress, consequently regulating important signaling pathways. Materials and methods: In this study, the effect of lipid raft disruption on AQP3, AQP5, and EFGR pathway was explored. Lipid rafts were disrupted bycholesterol depletion using methyl-β- cyclodextin. The effect of depletion was measured by MTT and BrdU incorporation assay, and the effects on EGFR signaling pathway was assayed by Western blot levels of pathway components. Results: Our results indicate that EGFR signaling pathway is affected by cholesterol depletion. Conclusions: We have managed to disrupt lipid rafts to interfere with EGFR signaling and are currently studying the consequences on signaling pathway components and levels of aquaporin 3 and aquaporin 5.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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