Pregled bibliografske jedinice broj: 1277098
The protective actions of DHEA/S and BDNF against oxidative stress in an in vitro model of vascular dementia
The protective actions of DHEA/S and BDNF against oxidative stress in an in vitro model of vascular dementia // FENS Forum 2022 : E-Book of abstracts
Pariz, Francuska, 2022. str. 4280-4280 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1277098 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The protective actions of DHEA/S and BDNF against oxidative stress in an in vitro model of vascular dementia
Autori
Vuić, Barbara ; Nikolac Perković, Matea ; Nedić Erjavec, Gordana ; Tudor, Lucija ; Miloš, Tina ; Pivac, Nela ; Konjevod, Marcela ; Švob Štrac, Dubravka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FENS Forum 2022 : E-Book of abstracts
/ - , 2022, 4280-4280
Skup
FENS Forum 2022 International Neuroscience Conference
Mjesto i datum
Pariz, Francuska, 09.07.2022. - 13.07.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
vascular dementia ; oxidative stress ; DHEA ; DHEAS ; BDNF
Sažetak
Vascular dementia (VaD), the second most common form of dementia, is generally underrecognized and still poorly understood disease. It develops when the brain´s blood supply is blocked or reduced, causing deprivation of oxygen and nutrients and resulting in damage and death of neurons. The brain is highly susceptible to oxidative stress, due to its richness in fatty acids sensitive to peroxidation, as well as due to high oxygen consumption and free radicals accumulation. Oxidative stress in VaD, characterized by the exacerbated production of reactive oxygen species and insufficient antioxidant defense system, increases neuronal cell abnormalities and triggers apoptosis, leading to cognitive dysfunction and dementia. Neurosteroids dehydroepiandrosterone and dehydroepiandrosterone sulfate (DHEA/S) and neurotrophin brain-derived neurotrophic factor (BDNF) have attracted the attention of researchers investigating their involvement in various brain functions such as neural survival, plasticity, cognition and behavior. The aim of this study was to investigate the protective potential of DHEA/S and BDNF in promoting neuronal survival and preventing oxidative stress caused by brain ischemia, a common characteristic of VaD. Oxygen-glucose deprivation (OGD) was performed in primary mouse neurons derived from C57BL/6 mice, and human SH-SY5Y neuroblastoma cells as in vitro model of VaD. The cells were cultured in glucose- and serum-free medium in a modular incubator chamber filled with N2. Before or after OGD, cells were treated with DHEA/S and BDNF and cell viability and oxidative stress parameters were determined. Our results suggested protective effects of DHEA/S and BDNF against oxidative damage induced by the ischemic injury in neuronal cells.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2019-04-6100 - Terapijski potencijal neurosteroida i neurotrofina u demenciji (TePoNeDe) (Švob Štrac, Dubravka, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Profili:
Marcela Konjevod
(autor)
Dubravka Švob Štrac
(autor)
Lucija Tudor
(autor)
Matea Nikolac Perković
(autor)
Gordana Nedić Erjavec
(autor)
Barbara Vuić
(autor)
Nela Pivac
(autor)
Tina Miloš
(autor)