Naslov
Metabolome profiling in the serum of dogs with chronic valvular disease using a targeted metabolomics approach and UHPLC-MS/MS

Autori
Mrljak, Vladimir ; Rubić, Ivana ; Rešetar Maslov, Dina ; Farkaš, Vladimir ; Kuleš, Josipa ; Barić Rafaj, Renata ; Beletić, Anđelo ; Torti, Marin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 21st European Congress of Internal Medicine (ECIM) / Huelgas, Ricardo Gomez ; Dicker, Dror - Atena, 2023, 581-581

Skup
21st European Congress of Internal Medicine (ECIM) joint with the 12th International Congress of Internal Medicine

Mjesto i datum
Atena, Grčka, 15.03.2023. - 18.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chronic valvular disease, serum, dogs, metabolomics

Sažetak
Background and Aims: Chronic valvular disease (CVD) is the most common heart disease in adult dogs. The CVD pathology is almost identical in humans and dogs. Therefore, canine CVD is considered a suitable animal model in human medicine. The aim of the study was to investigate serum metabolome of dogs with myxomatous mitral valve disease (MMVD) using a targeted metabolomics approach by UHPLC-MS/MS. Methods: Serum was collected from healthy dogs (N=7) and dogs with MMVD stages: B1 (N=7, with no/mild mitral regurgitation), B2 (N=9, advanced mitral regurgitation) and C (N=7, congestive heart failure). Samples were analyzed using the Absolute IDQ p400 kit on a Thermo Orbitrap Q Exactive Plus UHPLC-MS/MS. Metabolites were extracted from dog serum on the 96-well plate system for protein-removal, internal standard normalization and derivatization. Data processing was performed using the MetIDQ software. Results: Targeted metabolomics analysis of serum samples resulted in the identification of 154 metabolites divided into different groups: acylcarnitines (1), Biogenic amines (8), amino acids (18), sphingolipids (22), glycerides (21), cholesterol esters (5), glycerophospholipids (78) and sugars (1). Among them, 29 metabolites were significantly different between the four groups. The most significant metabolites were carnitine and sphingomyelin (38:1). Pathway analysis showed seven different pathways considered significant (p < 0.05, impact >0.1). Conclusions: Our results provide an overview of the serum metabolome of dogs with different CVD stages and highlight new targets for further investigation, as well as metabolites to serve as potential biomarkers for early detection of CVD and of progression from stage B1 to B2.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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