Naslov
Pathophysiological changes of the mice spinal cord after single and repetitive brain trauma

Autori
Janković Tamara ; Jaeger Marc ; Rajič Bumber Jelena ; Gržeta Nika ; Križ Jasna ; Dolenec Petra ; Pilipović Kristina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
MIND & BRAIN ; 62nd INTERNATIONAL NEUROPSYCHIATRIC CONGRESS / Demarin Vida ; Budinčević Hrvoje - , 2023, 63-64

Skup
MIND & BRAIN 62nd INTERNATIONAL NEUROPSYCHIATRIC CONGRESS

Mjesto i datum
Pula, Hrvatska, 18.05.2023. - 21.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
TDP-43, Spinal cord, Traumatic brain injury

Sažetak
Introduction/Objectives: Traumatic brain injury (TBI) occurs as a consequence of a mechanical force to the skull and causes life-long changes in the central nervous system (CNS). Neurodegenerative sequelae of TBI commonly include proteinopathies, such as the one involving the TAR DNA binding protein 43 (TDP-43). TDP-43 proteinopathy results in both gain of function, because of the formation of cytosolic aggregates that inhibit normal cellular mechanisms, and loss of function, with the lack of TDP-43 physiological properties. Repetitive brain traumas, which are common after sport and violent injuries, have been recognized as a risk factor for amyotrophic lateral sclerosis (ALS). There is less evidence of the association of a single brain trauma with TDP-43 proteinopathy. Studies have shown that brain injury can induce changes in the parts of the CNS that are distant from the initial trauma site, e.g., the hippocampus and cerebellum. The aim of this study was to investigate pathophysiological changes, including the ones related to the TDP-43 proteinopathy, of the mice spinal cord after single moderate and repetitive mild TBI. Participants, Materials/Methods: Single moderate brain trauma was induced over the left parietal cortex by the lateral fluid injury (LFPI) apparatus. A separate cohort of mice received repetitive mild TBI (RT) using the weight drop method, twice a day for five days in a row. Animals were sacrificed 14 days after LFPI or the last mild brain trauma and their spinal cords were prepared for immunohistological analyses. For both trauma models, sham-injured mice were used as the control group. Results: Cresyl violet staining did not reveal any significant morphological changes in the spinal cords of both trauma groups compared to their matching controls. Also, differences in the spinal cord white matter were not detected for RT and LFPI trauma groups of mice at this time point. The significant mislocalization of spinal cord TDP-43 was observed after both single moderate and repetitive mild brain injury. Cytoplasmic TDP-43 staining was evident in neurons but not in astrocytes of tested trauma groups. Conclusions: Our preliminary results suggest that both single and repetitive brain trauma can induce subtle pathophysiological changes, such as TDP-43 mislocalization, in CNS regions distant from the initial site of trauma. This work was supported by the University of Rijeka, Croatia, project uniri-biomed-18-199 to K.P. and uniri- biomed-18-204 to P.D.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA