Naslov
The effects of splenectomy in murine models of ischemic stroke: a systematic review and meta- analysis

Autori
Sternak, Marko ; Glasnović, Anton ; Josić, Paula ; Romić, Dominik ; Gajović, Srećko

Izvornik
Journal of Neuroinflammation (1742-2094) 19 (2022), 1; 233, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Splenectomy, Ischemic stroke, Monocytes, Infammation

Sažetak
Background: The spleen, a substantial reservoir of non-differentiated monocytes, may play a crucial role in the pathophysiology of post-ischemic inflammation and influence outcomes after ischemic stroke. Aim of the study: To analyze splenectomy as a preclinical intervention in murine models of ischemic stroke. Methods: Following systematic searches of PubMed, Scopus and Web of Science, a qualitative synthesis of study characteristics was performed, and the effect of splenectomy estimated by a three-level random-effects meta-analysis of infarct volumes and a conventional two-level random-effects meta- analysis of neurological deficit scores. Results: Database searches identified a total of 14 studies, 13 of which were used for meta- analysis. The ischemic lesion volumes were reduced in splenectomized animals compared to the control groups (difference in standardized mean differences: - 1.42 ; 95% CI [- 1.98, - 0.85] ; 95% PI [- 2.03, - 0.80] ; I2(2) = 19.04% ; 95% CI [0.00%, 65.49%] ; I2(3) = 47.24% ; 95% CI [0.00%, 85.23%]) and neurological deficit scores were improved (- 1.20 ; 95% CI [- 2.20, - 0.20] ; 95% PI [- 4.58, 2.18] ; I2 = 77.5% ; 95% CI [50.0%, 89.9%]). A subgroup analysis for infarct volumes showed that splenectomy performed prior to ischemia achieved a higher reduction of the ischemic lesion than when splenectomy was performed immediately prior or after stroke. Although the overall effect size of splenectomy could be classified as large, there was a significant presence of risks of bias, study heterogeneity, and a potential presence of publication bias. Conclusion: Despite limitations related to heterogeneity, risks of bias, and potential publication bias, this meta-analysis points to the spleen and its functional cell populations as promising targets for the therapeutic modulation of post-stroke inflammation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA