Naslov
Comparison of bioremediation strategies for the biodegradation of microplastics by bacteria and yeast

Autori
Miloloža, Martina ; Bule Možar, Kristina ; Ukić, Šime ; Bolanča, Tomislav ; Kučić Grgić, Dajana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Microbes in Industry and Environment / - , 2023, 39-39

Skup
Power of Microbes in Industry and Environment 2023

Mjesto i datum
Poreč, Hrvatska, 15.05.2023. - 18.05.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microplastics ; Delftia acidovorans ; Candida parapsilosis ; bacteria ; yeasts

Sažetak
Plastics have excellent application properties and therefore are used in various fields of human life. The annual production of plastics is increasing, as is the amount of plastic waste. Plastics are ubiquitous in the environment, where they break down into smaller particles called microplastics (MP) due to physical impact, UV radiation, and other environmental factors. Recently, ecotoxicological studies for MP have revealed potential adverse effects of MP on aquatic organisms. Therefore, it is necessary to investigate possible solutions to remove MP from the environment. In this context, the biodegradation of PS and PVC MP was studied using different microorganisms isolated from the environment enriched with MP. The experiments were divided into two parts: preliminary experiments and main experiments. The preliminary experiments on biodegradation of MP were performed with 5 bacteria (Bacillus cereus, Bacillus subtilis, Pseudomonas alcaligenes, Delftia acidovorans and Bacillus licheniformis) and 5 yeasts (Saccharomyces cerevisiae, Rhodotorula glutinis, Geotrichum candidum, Trichosporon sp. and Candida parapsilosis). During 30 days, the changes in the total number of bacteria and yeasts (CFU) were monitored. In addition, the particles of MP were analyzed by FTIR spectroscopy. Based on the results of the preliminary experiments, the most effective bacterium and yeast were selected for the main biodegradation experiment. Therefore, the main experiments with Delftia acidovorans and Candida parapsilosis were conducted according to a full factorial experimental design with 3 parameters at 3 levels to determine the optimal process conditions. The parameters studied were pH-value, agitation speed, and optical density of the bacteria/yeast suspension. In this case, the size of MP (25-100 μm), the concentration of MP (500 mg/L), and the temperature (25 ± 0.2 °C) were constant. In addition, the main objective of this work was to determine the most efficient bacterium and yeast for biodegradation of MP and the optimal conditions to achieve the highest efficiency of the biodegradation process.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Interdisciplinarne tehničke znanosti

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