Naslov
Plantaricin-based antibacterial activity of Lactiplantibacillus plantarum D13 and SF15C strains originating from different food microbiomes

Autori
Butorac, Katarina ; Novak, Jasna ; Banić, Martina ; Leboš Pavunc, Andreja ; Čuljak, Nina ; Šušković, Jagoda ; Kos, Blaženka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Microbes in Industry and Environment 2023 : Book of Abstract / Teparić, Renata ; Leboš Pavunc, Andreja ; Kifer, Domagoj - Zagreb : Hrvatsko mikrobiološko društvo, 2023, 85-85

ISBN
978-953-7778-19-4

Skup
Power of Microbes in Industry and Environment 2023

Mjesto i datum
Poreč, Hrvatska, 15.05.2023. - 18.05.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bacteriocin ; plantaricin ; probiotic ; antibacterial activity ; functional starter culture

Sažetak
In addition to being a rich source of nutrients, autochthonous fermented foods are a reservoir of beneficial lactic acid bacteria (LAB) starter cultures and potential probiotics. The bacteriocin-based antibacterial capacity of the autochthonous LAB is investigated as a strategy for biopreservation. Therefore, the aim here was to screen the antibacterial activity in a selected group of Lactiplantibacillus plantarum strains belonging to different food microbiomes, autochthonous cheese or sauerkraut. Since Listeria monocytogenes and Staphylococcus aureus are frequently detected as foodborne pathogens, they have been applied as test microorganisms to demonstrate antibacterial activities. All tested L. plantarum strains exhibited antilisterial and antistaphylococcal activities when grown on a solid medium, and when the cell-free supernatant (CFS) was analysed, where D13 and SF15C showed the greatest inhibition. Antibacterial activity in broth cultures was re-established or enhanced by coculturing with sensitive bacterial strains S. aureus 3048 and L. monocytogenes ATCC®19111™ which can be associated with the induction of bacteriocin production typically present among LAB. Additionally, the proteinase nature of CFSs has been confirmed as another feature of plantaricins. PCR using total DNA from L. plantarum strains resulted in the amplification of plantaricin-related genes plnA, plnEF and plnJ of expected amplicon size, suggesting that the genome could harbour a pln gene clusters. Whole genome sequencing (WGS) revealed the existence of five plantaricin specific operons regulated by a three- component regulatory system, involved in a quorum sensing (QS) mechanism, of L. plantarum D13 and SF15C strain, with high similarity to L. plantarum WCFS1. The 3D structures of PlnEF and PlnJK, were predicted using AlphaFold Protein Structure Database, while physicochemical properties and amino acid sequence of α-helixes of Pln were determined by HeliQuest analysis server. Taken together, these findings contribute to the characterisation of plantaricins of L. plantarum D13 and SF15C, and their implementation as either a functional starter cultures or even probiotics. Their application could improve the quality of fermented foods, which could be achieved by controlling contamination by L. monocytogenes, which would extend the shelf-life of the final product.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Nutricionizam

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