Pregled bibliografske jedinice broj: 1273907
The protective actions of DHEA/S and BDNF in an in vitro model of Parkinson's disease
The protective actions of DHEA/S and BDNF in an in vitro model of Parkinson's disease // Book of abstracts
Opatija, Hrvatska, 2022. str. 161-161 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 1273907 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The protective actions of DHEA/S and BDNF in an in
vitro model of Parkinson's disease
Autori
Miloš, Tina ; Vuić, Barbara ; Bacelj, Nora ; Nedić Erjavec, Gordana ; Tudor, Lucija ; Konjevod, Marcela ; Švob Štrac, Dubravka ; Nikolac Perković, M
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of abstracts
/ - , 2022, 161-161
Skup
10th Croatian Congress of Pharmacology and the 1st Croatian Congress of Clinical Pharmacology with international participation
Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Parkinson disease ; DHEA(S) ; BDNF ; therapy
Sažetak
Introduction: Parkinson's disease (PD) is the second most common neurodegenerative disorder that is characterized by the degeneration of dopaminergic neurons in the substantia nigra. Inducing an in vitro model of PD is a valuable tool for investigating the mechanisms involved in pathogenesis of disease, which is the key to identifying potential therapeutic strategies for PD. Rotenone and 6-hydroxydopamine are neurotoxins commonly used to generate the in vitro model of PD. Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS), and brain-derived neurotrophic factor (BDNF) are involved in neuroprotection and neuroregeneration, while their levels decrease with age and in neurodegenerative diseases such as PD. The aim of this study was to investigate potential neuroprotective actions of these agents in an in vitro model of PD. Materials and methods: Primary mouse neurons derived from C57BL/ 6 mice embryos and rat dopaminergic N27 cell line were injured with rotenone or 6-hydroxdopamine to induce in vitro model of PD. Both cell cultures were treated with DHEA(S) and BDNF, separately and combined, 16 h before injury. Alterations in cell viability were analyzed using the MTT test while oxidative stress parameters were determined using Cellular ROS Assay Kit 2', 7'-dichlorofluorescein diacetate (DCFHDA). Fluorescent dyes Hoechst 33342 and Propidium Iodide were used for staining apoptotic and necrotic cells. Results: Induced injury by rotenone or 6- hydroxydopamine showed lower metabolic activity and higher ROS levels, while DHEA(S), BDNF showed neuroprotective effect separately or combined on both cell cultures. Conclusion: Our results suggest that DHEA(S) and BDNF may play important role in prevention and treatment of PD.
Izvorni jezik
Engleski
POVEZANOST RADA
Profili:
Marcela Konjevod
(autor)
Gordana Nedić Erjavec
(autor)
Dubravka Švob Štrac
(autor)
Barbara Vuić
(autor)
Matea Nikolac Perković
(autor)
Lucija Tudor
(autor)
Tina Miloš
(autor)