Naslov
Neuroprotective effects of DHEA(S) and BDNF in an in vitro model of Parkinson's disease

Autori
Miloš, Tina ; Švob Štrac Dubravka ; Nikolac Perković Matea ; Tudor Lucija ; Nedić Erjavec Gordana ; Vuić Barbara ; Konjevod Marcela ; Pivac Nela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / - , 2022, 5336-5336

Skup
FENS Forum 2022

Mjesto i datum
Pariz, Francuska, 09.07.2022. - 13.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Parkinson diseases ; DHEA ; DHEAS ; BDNF

Sažetak
Parkinson’s disease (PD) is a neurodegenerative disease characterized by a selective loss of dopaminergic (DA) neurons and accumulation of α- synuclein in the substantia nigra. It is considered that gene mutations, environmental factors and aging are causative factors of PD. Understanding the mechanisms involved in neurodegeneration is the key to identifying potential therapeutic strategies for PD. Neurotoxins, such as rotenone and 6- hydroxidopamine are widely used to induce in vivo and in vitro model of PD. These neurotoxins induce mitochondrial dysfunction and increase oxidative stress, which leads to neuronal degeneration and cell death by apoptosis. Neurosteroids, dehydroepiandrosterone (DHEA) and its sulfate (DHEAS), are the most abundant steroid hormones and can be synthesized de novo in the brain. Levels of DHEA(S) decrease with age and play an important role in brain aging and neurodegeneration. Neurotrophin, brain-derived neurotrophic factor (BDNF) is also involved in neuroprotection and neuroregeneration, while decreased levels of BDNF were observed in the aging process and in neurodegenerative diseases such as PD. In this study we have investigated potential neuroprotective effects of DHEA(S) and BDNF in four different cell models: primary mouse neurons, SH-SY5Y neuroblastoma cells, PC12 and N27 cells. These cells were injured with rotenone or 6-hydroxidopamine to induce an in vitro model of PD. In addition, cells were treated with DHEA(S) and BDNF separately and combined to investigate their interactions and neuroprotective potential. The obtained results suggest that DHEA(S) and BDNF may play important role in prevention and treatment of PD.

Izvorni jezik
Engleski

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