Pregled bibliografske jedinice broj: 1272730
How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry
How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry // Clinical Microbiology and Infection, 24 (2018), 6; 599-603 doi:10.1016/j.cmi.2017.11.012 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1272730 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
How to: identify non-tuberculous Mycobacterium
species using MALDI-TOF mass spectrometry
Autori
Alcaide, F. ; Amlerová, J. ; Bou, G. ; Ceyssens, P.J. ; Coll, P. ; Corcoran, D. ; Fangous, M.-S. ; González-Álvarez, I. ; Gorton, R. ; Greub, G. ; Hery-Arnaud, G. ; Hrábak, J. ; Ingebretsen, A. ; Lucey, B. ; Marekoviċ, I. ; Mediavilla-Gradolph, C. ; Monté, M.R. ; O'Connor, J. ; O'Mahony, J. ; Opota, O. ; O'Reilly, B. ; Orth-Höller, D. ; Oviaño, M. ; Palacios, J.J. ; Palop, B. ; Pranada, A.B. ; Quiroga, L. ; Rodríguez-Temporal, D. ; Ruiz-Serrano, M.J. ; Tudó, G. ; Van den Bossche, A. ; van Ingen, J. ; Rodriguez-Sanchez, B.
Izvornik
Clinical Microbiology and Infection (1198-743X) 24
(2018), 6;
599-603
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Identification ; MALDI-TOF MS ; Non-tuberculous mycobacteria ; Procedure ; Sample preparation
Sažetak
Background: The implementation of MALDI-TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably identified within minutes using this inexpensive, user-friendly methodology. However, its application in the identification of mycobacteria isolates has been hampered by the structure of their cell wall. Improvements in the sample processing method and in the available database have proved key factors for the rapid and reliable identification of non-tuberculous mycobacteria isolates using MALDI-TOF MS. Aims: The main objective is to provide information about the proceedings for the identification of non-tuberculous isolates using MALDI-TOF MS and to review different sample processing methods, available databases, and the interpretation of the results. Sources: Results from relevant studies on the use of the available MALDI-TOF MS instruments, the implementation of innovative sample processing methods, or the implementation of improved databases are discussed. Content: Insight about the methodology required for reliable identification of non-tuberculous mycobacteria and its implementation in the microbiology laboratory routine is provided. Implications: Microbiology laboratories where MALDI-TOF MS is available can benefit from its capacity to identify most clinically interesting non-tuberculous mycobacteria in a rapid, reliable, and inexpensive manner.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb
Profili:
Ivana Mareković
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE