Naslov
UPDATE ON BOTULINUM TOXIN TYPE A CENTRAL ACTION ON PAIN – ARE THE PRIMARY AFFERENTS IN THE SPINAL CORD ITS FINAL DESTINATION?

Autori
Vađunec Dalia, Matak Ivica, Bach-Rojecky Lidija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Pharmaca 2022, 52 Suppl 2: 133 / - , 2022, 133-133

Skup
10. HRVATSKI KONGRES FARMAKOLOGIJE I 1. HRVATSKI KONGRES KLINIČKE FARMAKOLOGIJE

Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Recenziran

Ključne riječi
pain, botulinum toxin type A, transcytosis, rats

Sažetak
Introduction The mechanism of botulinum toxin type A (BoNT-A) antinociceptive effect in the central nervous system is yet to be clarified. This study aimed to investigate possibility of BoNT-A transcytosis at the level of the spinal cord after its peripheral application in rats. Materials and methods Male Wistar rats were unilaterally injected into the plantar surface of the hind paws (i.pl.) with BoNT-A (7 IU/kg) one day before intrathecal administration of antitoxin against BoNT-A (2 IU/10μL). After six days carrageenan solution (2%) was injected i.pl. bilaterally and three hours later sensitivity to mechanical stimuli was measured. Animals were sacrificed and immunohistochemistry of c-Fos, (marker of neural activation) and cleaved SNAP-25 (marker of BoNT-A biological activity) were performed in the lumbar spinal cord sections. Experiments were approved by Ethical Committee of University of Zagreb School of Medicine. Results Peripheral unilateral BoNT-A significantly reduced the mechanical hypersensitivity on both, ipsilateral and contralateral paws. Antitoxin prevented this effect of BoNT-A on both sides. Immunohistochemical data showed reduction of c-Fos positive neurons bilaterally in dorsal horn at L4/L5 segments in BoNT-A pre-treated animals, which was also prevented by antitoxin. Additionally, quantification of cleaved SNAP-25- positive neurons showed strong signal on both sides of the dorsal horns of BoNT-A-treated rats, which was significantly lower in antitoxin-treated group. Conclusion Here we provide evidence for complex BoNT-A’s central action on pain, which probably involves the toxin transsynaptic transport within the spinal cord. Further in-depth studies are needed to elucidate this possibility. This research is supported by Croatian Science Foundation (project no. HRZZ-UIP-2019-04- 8277).

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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