Pregled bibliografske jedinice broj: 1271452
The safety of botulinum neurotoxin type A's intraarticular application in experimental animals.
The safety of botulinum neurotoxin type A's intraarticular application in experimental animals. // Toxicon X, 18 (2023), 100155, 9 doi:10.1016/j.toxcx.2023.100155 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1271452 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The safety of botulinum neurotoxin type A's
intraarticular application in experimental animals.
Autori
Okroša Dugonjić, Ana ; Muñoz-Lora, Victor ; Matak, Ivica ; Bach-Rojecky, Lidija ; Kalinichev, Mikhail ; Lacković, Zdravko
Izvornik
Toxicon X (2590-1710) 18
(2023);
100155, 9
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
abobotulinumtoxinA, onabotulinumtoxinA, Systemic spread, Motor effects, Rats
(abobotulinumtoxinA onabotulinumtoxinA Systemic spread Motor effects Rats)
Sažetak
In vivo studies of botulinum neurotoxin type A (BoNT-A) enabled characterization of its activity in the nociceptive sensory system separate from its preferred action in motor and autonomic nerve terminals. However, in the recent rodent studies of arthritic pain which employed high intra- articular (i.a.) doses (expressed as a total number of units (U) per animal or U/kg), possible systemic effects have not been conclusively excluded. Herein we assessed the effect of two pharmaceutical preparations, abobotulinumtoxinA (aboBoNT-A, 10, 20, and 40 U/kg corresponding to 0.05, 0.11, and 0.22 ng/kg neurotoxin) and onabotulinumtoxinA (onaBoNT-A, 10 and 20 U/ kg corresponding to 0.09 and 0.18 ng/kg, respectively) injected into the rat knee, on safety-relevant readouts: digit abduction, motor performance and weight gain during 14 days post- treatment. The i. a. toxin produced dose-dependent impairment of the toe spreading reflex and rotarod performance, which was moderate and transient after 10 U/kg onaBoNT-A and ≤20 U/kg aboBoNT-A doses, and severe and long-lasting (examined up to 14 days) after ≥20 U/kg of onaBoNT-A and 40 U/kg aboBoNT-A. In addition, lower toxin doses prevented the normal weight gain compared to controls, while higher doses induced marked weight loss (≥20 U/kg of onaBoNT-A and 40 U/kg aboBoNT- A). Commonly employed BoNT-A formulations, depending on the doses, cause local relaxation of the surrounding muscles and systemic adverse effects in rats. Thus, to evade possible toxin unwanted local or systemic spread, careful dosing and motor testing should be mandatory in preclinical behavioral studies, irrespective of the sites and doses of toxin application.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Farmacija
POVEZANOST RADA
Projekti:
UIP-2019-04-8277 - Motorički učinci klostridijskih neurotoksina u središnjem živčanom sustavu (MEFCLO) (Matak, Ivica, HRZZ - 2019-04) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb
Profili:
Ana Dugonjić Okroša
(autor)
Zdravko Lacković
(autor)
Lidija Bach Rojecky
(autor)
Ivica Matak
(autor)