Chitosan/collagen/Mg, Se, Sr, Zn-substituted calcium phosphate scaffolds for bone tissue engineering applications: A growth factor free approach

Ressler, Antonia; Ohlsbom, Roope; Žužić, Andreja; Gebraad, Arjen; Frankberg, Erkka J.; Pakarinen, Toni-Karri; Ivanković, Hrvoje; Miettinen, Susanna; Ivanković, Marica

doi:10.1016/j.eurpolymj.2023.112129

Pregled bibliografske jedinice broj: 1271393

Chitosan/collagen/Mg, Se, Sr, Zn-substituted calcium phosphate scaffolds for bone tissue engineering applications: A growth factor free approach

Ressler, Antonia; Ohlsbom, Roope; Žužić, Andreja; Gebraad, Arjen; Frankberg, Erkka J.; Pakarinen, Toni-Karri; Ivanković, Hrvoje; Miettinen, Susanna; Ivanković, Marica

Chitosan/collagen/Mg, Se, Sr, Zn-substituted calcium phosphate scaffolds for bone tissue engineering applications: A growth factor free approach // European polymer journal, 194 (2023), 112129, 14 doi:10.1016/j.eurpolymj.2023.112129 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1271393 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Chitosan/collagen/Mg, Se, Sr, Zn-substituted calcium phosphate scaffolds for bone tissue engineering applications: A growth factor free approach

Autori
Ressler, Antonia ; Ohlsbom, Roope ; Žužić, Andreja ; Gebraad, Arjen ; Frankberg, Erkka J. ; Pakarinen, Toni-Karri ; Ivanković, Hrvoje ; Miettinen, Susanna ; Ivanković, Marica

Izvornik
European polymer journal (0014-3057) 194 (2023); 112129, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Chitosan ; Collagen ; Ionic substitution ; Lysozyme ; Osteogenic differentiation ; Scaffold ; Regeneration

Sažetak
According to the biomimetic bone scaffold design paradigm, a scaffold resembling natural bone tissue with molecular, structural and biological compatibility is needed to allow effective regeneration of bone tissue. Continuing our previous studies regarding scaffolds with chitosan matrix containing Mg, Se, Sr, Zn-substituted calcium phosphates (CaPs), the focus of this work was to further improve the properties of these growth factor-free scaffolds.By addition of collagen into the chitosan matrix at weight ratios of 100:0, 75:25, 50:50, 25:75 and 0:100, we aimed to better resemble natural bone tissue. Highly porous composite scaffolds based on chitosan and collagen, with 30 wt% of Mg, Se, Sr, Zn-substituted CaPs, were prepared by the freeze-gelation method. The scaffolds show a highly porous structure, with interconnected pores in the range of 20–350 μm and homogeneously dispersed CaPs. The added collagen further enhanced the stability measured during 28 days in simulated biological conditions. Live/dead and CyQUANT assays confirmed good viability and proliferation of human bone marrow-derived mesenchymal stem/stromal cells, while successful osteogenic differentiation was confirmed by alkaline phosphatase quantification and type I collagen immunocytochemical staining. Results indicated that the addition of collagen into the chitosan matrix containing Mg, Se, Sr, Zn-substituted CaPs improved the physicochemical and biological properties of the scaffolds.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Temeljne tehničke znanosti, Interdisciplinarne tehničke znanosti

POVEZANOST RADA

Projekti:
EK-EFRR-KK.01.1.1.07.0014 - Biorazgradive 3D tiskane strukture za augmentaciju kosti (BIDEAS) (Ivanković, Hrvoje, EK - KK.01.1.1.07) ( CroRIS)
EK-EFRR-KK.01.1.1.02.0022 - Virtulab – Integrirani laboratorij za primarne i sekundarne sirovine (Virtulab) (Borojević Šoštarić, Sibila; Šantek, Božidar; Rogošić, Marko; Petrović, Igor; Zovko Brodarac, Zdenka, EK - KK.01.1.1.02) ( CroRIS)

Profili:

Andreja Žužić (autor)