Naslov
UTJECAJ 2-METOKSI-1,4- NAFTOKINONA NA KLIJANJE I PARAMETRE OKSIDACIJSKOG STRESA BIJELE GORUŠICE
(THE EFFECT OF 2-METHOXY-1,4-NAPHTHOQINONE ON GERMINATION AND OXIDATIVE STRESS PARAMETERS OF WHITE MUSTARD)

Autori
Fajdetić, Ana ; Kenfelj, Ivan ; Vujčić Bok, Valerija ; Maleš, Željan ; Domijan, Ana-Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 4th International ZORH Conference / Sedlar, Jelena ; Alešković, Luka Marijan ; Nižić, Katarina - Split, 2023, P6-P6

ISBN
978-953-7803-20-9

Skup
4th International ZORH Conference

Mjesto i datum
Split, Hrvatska, 20.04.2023. - 21.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
naphthoquinones ; Sinapis alba L. ; glutathione ; malondialdehyde ; superoxide dismutase

Sažetak
The 2-methoxy-1, 4-naphthoquinone (2-MNQ, lawsone methyl ether) is chemical compound found in plants and microorganisms with diverse biological activities (antibacterial, antifungal, antiviral, and antitumor) [1]. The aim of this study was to investigate the impact of 2-MNQ on germination and oxidative stress parameters of white mustard (Sinapis alba L.). The seeds of white mustard (n = 25) were placed in Petri dishes on filter paper impregnated with 2-MNQ at the following concentrations: 0, 1, 5, 10, 20, 30 and 40 µg/mL and germinated in dark at room temperature for three days. Each concentration was tested in triplicate and 0.02 M CuSO4 was used as positive control. After germination, the seedlings were weighed, and roots length were measured. For spectrophotometric assessment of oxidative stress parameters: superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) plant tissue homogenates were prepared. Significant differences between treatments were tested by one- way ANOVA followed by Duncan's multiple range test. Germination of white mustard seeds was significantly reduced after exposure to 5 µg/mL of 2-MNQ while exposure to 2-MNQ at concentrations 30 and 40 µg/mL, and positive control complete ceased germination of white mustard. The reduction in fresh tissue weight and root length of white mustard seedlings was observed already after exposure to 1 µg/mL of 2-MNQ. Decrease in MDA content was detected in seedlings exposed to 10 and 20 µg/mL of 2-MNQ. The lowest 2-MNQ concentration (1 µg/mL) significantly reduced GSH level of white mustard seedlings, while 2-MNQ at concentrations 5-20 µg/mL significantly increased GSH levels. Significant increase in SOD activity was detected in white mustard seedlings exposed to 1 and 10 µg/mL of 2-MNQ. It can be concluded that 2-MNQ is phytotoxic to white mustard and that oxidative stress is involved in its phytotoxic effect. [1] Ž. Maleš, A.-M. Domijan, I. Duka, T. Marić, M. Bojić, H. Rimac, B. Mitić, D. Hruševar, K. Barišić and D. Verbanac. Determination of naphthoquinones in invasive alien plants Impatiens glandulifera Royle and I. balfourii Hook. from Croatia. Croatica Chemica Acta, Vol. 95, 2022, 1-5.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija

POVEZANOST RADA