Naslov
UTJECAJ BISFENOLA A NA PARAMETRE OKSIDACIJSKOG STRESA U LUKOVICAMA LUKA
(INFLUENCE OF BISPHENOL A ON OXIDATIVE STRESS PARAMETERS IN ONION BULBS)

Autori
Režan, Luka ; Vujčić Bok, Valerija ; Gagić, Sanja ; Domijan, Ana-Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 4th International ZORH Conference / Sedlar, Jelena ; Alešković, Luka Marijan ; Nižić, Katarina - Split, 2023, P5-P5

ISBN
978-953-7803-20-9

Skup
4th International ZORH Conference

Mjesto i datum
Split, Hrvatska, 20.04.2023. - 21.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Allium cepa L. ; bisphenol A ; malondialdehyde ; reactive oxygen species ; superoxide dismutase

Sažetak
Bisphenol A (BPA) is used as intermediate in production of plastic-based materials. Since plastic materials are widely used in daily life, the amount of BPA in the environment is increasing. Therefore, it is necessary to investigate the effects of BPA on environment, especially on terrestrial plants [1]. The aim of this study was to test whether oxidative stress is mechanism of BPA toxicity. Onion (Allium cepa L.) was used as plant model. Onion roots were treated with BPA at concentrations of 1, 5, 10, 25 and 50 mg/L for three days. After treatment, onion bulbs were collected, oven dried (at 60 °C), and homogenized in PBS buffer (50 mg/mL). The homogenates were centrifuged (10000 g for 10 min) and oxidative stress parameters were measured in supernatant. The level of malondialdehyde (MDA), as a marker of lipid peroxidation, was determined spectrophotometrically with thiobarbituric acid and reactive oxygen species (ROS) were followed with fluorescent probes: H2O2 with fluorescent probe 2’, 7’-dichlorofluorescin diacetate and O2 with fluorescent probe dihydroethidium. Antioxidative defense was determined by assessing superoxide dismutase (SOD) activity using commercial kit (Cayman Chemical, Ann Arbor, MI, USA). All measurements were performed on microplate reader (SpectraMax i3x, Molecular Devices, San Jose, CA, USA). Significant differences between treatments were examined using a one-way test (ANOVA) followed by Duncan's multiple range test. In onion bulbs significant increase in MDA content was observed already after the treatment with BPA at concentration of 5 mg/L. The same BPA concentration (5 mg/L) resulted in a significant increase in H2O2 and O2 level. The decrease in SOD activity was recorded after treatment with the lowest BPA concentration (1 mg/L) indicating that even the lowest applied BPA concentration causes a change in antioxidative defense. From the obtained results it can be concluded that BPA induces changes in oxidative stress parameters, suggesting that oxidative stress is mechanism of BPA phytotoxicity. Funding: Bilateral collaboration projects between Republic of Slovenia and Republic of Croatia (BI- HR/18-19-003 ; BI-HR/20-21-031) ; University of Zagreb ; European Regional Development Fund project FarmInova (KK.01.1.1.02.0021). [1] V. Vujčić Bok, M. Gerić, G. Gajski, S, Gagić and A.-M. Domijan. Phytotoxicity of Bisphenol A to Allium cepa Root Cells Is Mediated through Growth Hormone Gibberellic Acid and Reactive Oxygen Species. Molecules. Vol. 28, 2023, 2046, 1-15.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija

POVEZANOST RADA