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Pregled bibliografske jedinice broj: 1270092

Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?


Kerep, Robert; Šeba, Tino; Borko, Valentina; Weitner, Tin; Keser, Toma; Lauc, Gordan; Gabričević, Mario
Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics? // International Journal of Molecular Sciences, 24 (2023), 10; 8472, 13 doi:10.3390/ijms24108472 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1270092 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?

Autori
Kerep, Robert ; Šeba, Tino ; Borko, Valentina ; Weitner, Tin ; Keser, Toma ; Lauc, Gordan ; Gabričević, Mario

Izvornik
International Journal of Molecular Sciences (1422-0067) 24 (2023), 10; 8472, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
alpha-1 acid glycoprotein ; drugs ; plasma protein binding ; sialic acid ; binding affinity ; isothermal titration calorimetry

Sažetak
Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances. It has been reported that the sialic acid groups that terminate the N–glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein. The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs—clindamycin, diltiazem, lidocaine, and warfarin—was quantitatively evaluated using isothermal titration calorimetry. The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction. The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10−5–10−6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference. Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija



POVEZANOST RADA


Projekti:
IP-2016-06-3672 - Glikozilacija alfa kiselog glikoproteina - put prema personaliziranoj terapiji (GlycoDrugs) (Gabričević, Mario, HRZZ - 2016-06) ( CroRIS)
EK-EFRR-KK.01.1.1.02.0021 - Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu (FarmInova) (Lovrić, Jasmina, EK - Ulaganje u organizacijsku reformu i infrastrukturu u sektoru istraživanja, razvoja i inovacija) ( CroRIS)
UIP-2017-05-9537 - Glikozilacija serumskog transferina kao faktor u mehanizmu prijenosa željeza (GlyMech) (Weitner, Tin, HRZZ - 2017-05) ( CroRIS)
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Polašek, Ozren; Secenji, Aleksandar, EK ) ( CroRIS)
--KK.01.1.1.07.0055 - Razvoj metoda za proizvodnju i obilježavanje glikanskih standarda za molekularnu dijagnostiku (Gabričević, Mario) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb

Profili:

Avatar Url Valentina Borko (autor)

Avatar Url Mario Gabričević (autor)

Avatar Url Tino Šeba (autor)

Avatar Url Robert Kerep (autor)

Avatar Url Gordan Lauc (autor)

Avatar Url Tin Weitner (autor)

Avatar Url Toma Keser (autor)

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Kerep, Robert; Šeba, Tino; Borko, Valentina; Weitner, Tin; Keser, Toma; Lauc, Gordan; Gabričević, Mario
Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics? // International Journal of Molecular Sciences, 24 (2023), 10; 8472, 13 doi:10.3390/ijms24108472 (međunarodna recenzija, članak, znanstveni)
Kerep, R., Šeba, T., Borko, V., Weitner, T., Keser, T., Lauc, G. & Gabričević, M. (2023) Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?. International Journal of Molecular Sciences, 24 (10), 8472, 13 doi:10.3390/ijms24108472.
@article{article, author = {Kerep, Robert and \v{S}eba, Tino and Borko, Valentina and Weitner, Tin and Keser, Toma and Lauc, Gordan and Gabri\v{c}evi\'{c}, Mario}, year = {2023}, pages = {13}, DOI = {10.3390/ijms24108472}, chapter = {8472}, keywords = {alpha-1 acid glycoprotein, drugs, plasma protein binding, sialic acid, binding affinity, isothermal titration calorimetry}, journal = {International Journal of Molecular Sciences}, doi = {10.3390/ijms24108472}, volume = {24}, number = {10}, issn = {1422-0067}, title = {Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?}, keyword = {alpha-1 acid glycoprotein, drugs, plasma protein binding, sialic acid, binding affinity, isothermal titration calorimetry}, chapternumber = {8472} }
@article{article, author = {Kerep, Robert and \v{S}eba, Tino and Borko, Valentina and Weitner, Tin and Keser, Toma and Lauc, Gordan and Gabri\v{c}evi\'{c}, Mario}, year = {2023}, pages = {13}, DOI = {10.3390/ijms24108472}, chapter = {8472}, keywords = {alpha-1 acid glycoprotein, drugs, plasma protein binding, sialic acid, binding affinity, isothermal titration calorimetry}, journal = {International Journal of Molecular Sciences}, doi = {10.3390/ijms24108472}, volume = {24}, number = {10}, issn = {1422-0067}, title = {Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?}, keyword = {alpha-1 acid glycoprotein, drugs, plasma protein binding, sialic acid, binding affinity, isothermal titration calorimetry}, chapternumber = {8472} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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