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Pregled bibliografske jedinice broj: 1269810

Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro


Delić, Luka; Matković, Katarina; Rinkovec, Jasmina; Jakovljević , Ivana; Pehnec, Gordana; Domijan, Ana-Marija; Šišková, Anna; Gajski, Goran; Gerić, Marko
Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro // Book of Abstracts 4th International ZORH Conference
Split, Hrvatska, 2023. str. 8-8 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1269810 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro

Autori
Delić, Luka ; Matković, Katarina ; Rinkovec, Jasmina ; Jakovljević , Ivana ; Pehnec, Gordana ; Domijan, Ana-Marija ; Šišková, Anna ; Gajski, Goran ; Gerić, Marko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 4th International ZORH Conference / - , 2023, 8-8

Skup
4th International ZORH Conference

Mjesto i datum
Split, Hrvatska, 20.04.2023. - 21.04.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
air pollution, polycyclic aromatic hydrocarbons, toxic metals, cytogenotoxicity, human peripheral blood cells

Sažetak
Air pollution remains a major issue in environmental and public health and has been recognized by leading world authorities as a risk factor associated with adverse health outcomes. In addition to cancer, exposure to air pollutants has been linked to the onset of cardiovascular and respiratory disease, as well as premature mortality. The most important pollutants to note are particulate matter (PM), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOC), and toxic metals. Therefore, we aimed to investigate the possible cytogenotoxic effects of a complex mixture of PAHs and toxic metals in vitro in a range of concentrations of air pollutants from 0.5 to 50 cm3/mL. The mixture was prepared based on the outdoor air pollution monitoring data in Zagreb (Croatia) collected during our human biomonitoring study in the colder period of the year 2021 representing the translation of real-scenario exposure to in vitro. Cytogenotoxicity was evaluated on human peripheral blood cells employing the cell viability assay and the alkaline comet assay intended for the assessment of primary DNA damage to better understand the mechanism of action of the tested mixture. Based on the obtained results, we did not observe any impact of the complex mixture on cell viability that remained similar compared to the non-treated cells. As for the DNA damaging effect, only the highest tested concertation (50 cm3/mL) was able to increase primary strand breaks as measured by the % tail DNA as the comet assay descriptor. Our results indicate the necessity of testing complex mixtures that may pose a risk to cell and genome integrity in a complex environment, especially at low environmentally relevant concentrations. Since oxidative stress and inflammation are suspected mechanisms of action of air pollution, further research will include various biomarkers of oxidative stress as well as other biomarkers of genomic instability in order to avoid any possible adverse effects on the environment and human health.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb


Citiraj ovu publikaciju:

Delić, Luka; Matković, Katarina; Rinkovec, Jasmina; Jakovljević , Ivana; Pehnec, Gordana; Domijan, Ana-Marija; Šišková, Anna; Gajski, Goran; Gerić, Marko
Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro // Book of Abstracts 4th International ZORH Conference
Split, Hrvatska, 2023. str. 8-8 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Delić, L., Matković, K., Rinkovec, J., Jakovljević , I., Pehnec, G., Domijan, A., Šišková, A., Gajski, G. & Gerić, M. (2023) Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro. U: Book of Abstracts 4th International ZORH Conference.
@article{article, author = {Deli\'{c}, Luka and Matkovi\'{c}, Katarina and Rinkovec, Jasmina and Jakovljevi\'{c}, Ivana and Pehnec, Gordana and Domijan, Ana-Marija and \v{S}i\v{s}kov\'{a}, Anna and Gajski, Goran and Geri\'{c}, Marko}, year = {2023}, pages = {8-8}, keywords = {air pollution, polycyclic aromatic hydrocarbons, toxic metals, cytogenotoxicity, human peripheral blood cells}, title = {Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro}, keyword = {air pollution, polycyclic aromatic hydrocarbons, toxic metals, cytogenotoxicity, human peripheral blood cells}, publisherplace = {Split, Hrvatska} }
@article{article, author = {Deli\'{c}, Luka and Matkovi\'{c}, Katarina and Rinkovec, Jasmina and Jakovljevi\'{c}, Ivana and Pehnec, Gordana and Domijan, Ana-Marija and \v{S}i\v{s}kov\'{a}, Anna and Gajski, Goran and Geri\'{c}, Marko}, year = {2023}, pages = {8-8}, keywords = {air pollution, polycyclic aromatic hydrocarbons, toxic metals, cytogenotoxicity, human peripheral blood cells}, title = {Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro}, keyword = {air pollution, polycyclic aromatic hydrocarbons, toxic metals, cytogenotoxicity, human peripheral blood cells}, publisherplace = {Split, Hrvatska} }




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