Pregled bibliografske jedinice broj: 1269810
Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro
Understanding air pollution impact at the cellular level: translation of real-scenario exposure to in vitro // Book of Abstracts 4th International ZORH Conference
Split, Hrvatska, 2023. str. 8-8 (predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1269810 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Understanding air pollution impact at the cellular
level: translation of real-scenario exposure to in
vitro
Autori
Delić, Luka ; Matković, Katarina ; Rinkovec, Jasmina ; Jakovljević , Ivana ; Pehnec, Gordana ; Domijan, Ana-Marija ; Šišková, Anna ; Gajski, Goran ; Gerić, Marko
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts 4th International ZORH Conference
/ - , 2023, 8-8
Skup
4th International ZORH Conference
Mjesto i datum
Split, Hrvatska, 20.04.2023. - 21.04.2023
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
air pollution, polycyclic aromatic hydrocarbons, toxic metals, cytogenotoxicity, human peripheral blood cells
Sažetak
Air pollution remains a major issue in environmental and public health and has been recognized by leading world authorities as a risk factor associated with adverse health outcomes. In addition to cancer, exposure to air pollutants has been linked to the onset of cardiovascular and respiratory disease, as well as premature mortality. The most important pollutants to note are particulate matter (PM), polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds (VOC), and toxic metals. Therefore, we aimed to investigate the possible cytogenotoxic effects of a complex mixture of PAHs and toxic metals in vitro in a range of concentrations of air pollutants from 0.5 to 50 cm3/mL. The mixture was prepared based on the outdoor air pollution monitoring data in Zagreb (Croatia) collected during our human biomonitoring study in the colder period of the year 2021 representing the translation of real-scenario exposure to in vitro. Cytogenotoxicity was evaluated on human peripheral blood cells employing the cell viability assay and the alkaline comet assay intended for the assessment of primary DNA damage to better understand the mechanism of action of the tested mixture. Based on the obtained results, we did not observe any impact of the complex mixture on cell viability that remained similar compared to the non-treated cells. As for the DNA damaging effect, only the highest tested concertation (50 cm3/mL) was able to increase primary strand breaks as measured by the % tail DNA as the comet assay descriptor. Our results indicate the necessity of testing complex mixtures that may pose a risk to cell and genome integrity in a complex environment, especially at low environmentally relevant concentrations. Since oxidative stress and inflammation are suspected mechanisms of action of air pollution, further research will include various biomarkers of oxidative stress as well as other biomarkers of genomic instability in order to avoid any possible adverse effects on the environment and human health.
Izvorni jezik
Engleski
Znanstvena područja
Javno zdravstvo i zdravstvena zaštita
POVEZANOST RADA
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb
Profili:
Ana-Marija Domijan (autor)
Goran Gajski (autor)
Luka Delić (autor)
Katarina Matković (autor)
Marko Gerić (autor)
Ivana Jakovljević (autor)
Jasmina Rinkovec (autor)
Gordana Pehnec (autor)