Naslov
Changes in the microbiome of a successful freshwater invader along the environmental gradient
(Changes in the microbiome of a successful freshwater invader along the environmental gradient)

Autori
Dragičević, Paula ; Bielen, Ana ; Žučko, Jurica ; Sviličić Petrić, Ines ; Hudina, Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of 4th Symposium of Freshwater Biology / Mičetić Stanković, Vlatka ; Ivković, Marija ; Matoničkin Kepčija, Renata ; Sertić Perić, Mirela ; Miliša, Marko ; Vilenica, Marina - Zagreb : Hrvatsko udruženje slatkovodnih ekologa (HUSEk), 2023, 16-16

Skup
4th Symposium of Freshwater Biology

Mjesto i datum
Zagreb, Hrvatska, 21.04.2023

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bacteriome ; mycobiome ; amplicon sequencing ; microenvironment ; crayfish

Sažetak
The introduction of invasive alien species into sensitive aquatic ecosystems contributes to the loss of freshwater biodiversity by altering ecosystem structure and services, and by transferring new, potentially unfamiliar microbial pathogens to native species. However, the microbes in the invaded ecosystem also interact with the microbiome of the invader, which can affect invader’s physiology, immune status, health, and fitness. Additionally, these interactions between microbes can also be affected by changing environmental conditions, further affecting the invader and ultimately the invasion success. We analyzed the bacteriome and mycobiome of the signal crayfish, Pacifastacus leniusculus, one of the most successful freshwater invertebrate invaders in Europe. We collected the signal crayfish individuals, water and sediment samples, along this species' invasion range in the Korana River, Croatia. Using 16S and ITS rRNA amplicon sequencing, we examined the microbiomes of different crayfish tissues (exoskeleton, hemolymph, hepatopancreas, intestine), and environment (water, sediment). We investigated the differences in these microbiomes between two different microenvironments in the Korana River, i.e. upstream and downstream river segments. Our results showed significant differences between all crayfish and environmental microbiomes, confirming their uniqueness. Generally, the environmental microbiomes, and the crayfish exoskeletal microbiome (which is in continuous contact with the environment), exhibited higher taxonomic richness than the microbiomes of internal crayfish tissues. The exoskeletal and hemolymph microbiomes differed between two river segments, indicating that they are at least partly shaped by the environment. Our results provide insight into the microbiome of a successful crayfish invader and report its changes during dispersal through different microenvironments.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA