Naslov
ANTIOXIDANT, ANTI-INFLAMMATORY, AND BIOCOMPATIBILITY EFFECT OF CYCLODEXTRIN-GLYCEROL BASED EXTRACTS OF SATUREJA MONTANA

Autori
Jakupović, Lejsa ; Marijan, Marijan ; Cvetko, Josip, Marketin, Blanka ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of 7th PhD Student Symposium / - , 2023, 110-110

Skup
7th PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 20.04.2023. - 21.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
satureja montana, antioxidant, anti-inflammatory activity, skin, glycerol-cyclodextrin extracts, nanoparticles

Sažetak
Winter savory (Satureja montana L.) is annual, medicinal, and aromatic plant from the Lamiaceae family. It is a plant which grows in sub- Mediterranean areas in dry, sunny, and rocky habitats [1]. In Mediterranean diet, Satureja montana is widely used for its anti-inflammatory, antibacterial, antifungal and antioxidant properties. In this work, antioxidant, anti-inflammatory and biocompatibility effects of glycerol-cyclodextrin based extracts of S.montana were investigated. The extracts were prepared using biocompatible solvents, such as glycerol and water with addition of 2- hydroxypropy-β-cyclodextrin. The free-radical- scavenging activity (RSA) of both extracts was assessed with the 2, 2-diphenyl-1-pycrylhydrazyl (DPPH) method. The anti-inflammatory activity was investigated by using anti-lipoxygenase (anti-LOX) assays and the heat-induced ovalbumin coagulation method [2]. Biocompatibility effect was investigated by using MTT assay on the HaCaT cells [3]. The extracts (SMCD0 ; SMCD015 ; SMCD020 ; SMCD034) showed strong antioxidant activity (2.07 ± 0.05 μg/mL, 1.69 ± 0.31 μg/mL, 1.4 ± 0.14 μg/mL, 1.22 ± 0 μg/mL, respectively) and were significant greater compered to positive control (butylated hydroxyanisole (BHA) (8.32 ± 0.11 μg/mL). In both anti- inflammatories performed assays, heat-induced ovalbumin and LOX inhibition assay, the extract SMCD020 (26.98 ± 3.81 μg/mL ; 2.56 ± 0.19 μg/mL, respectively) showed the best activity between extracts and it was significantly greater compered to positive control (diclofenac: 248.81 ± 8.27 μg/mL ; NDGA: 7.02 ± 0.57 μg/mL). The MTT-assay was performed in concentration interval between 8 – 250 μL/mL. All extracts showed high cell viability in lower concentrations (8 – 125 μL/mL). The observed antioxidant-, enzyme-inhibiting and biocompatibility activity of S.montana extracts prepared using environmentally friendly and non-toxic solvents makes them promising ingredients for natural cosmetics products with potential anti- inflammatory effects.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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