Naslov
Unique and overlapping transcriptional targets of GLI1, GLI2 and GLI3 in melanoma cell lines

Autori
Kurtović, Matea ; Bartoniček, Nenad ; Piteša, Nikolina ; Ozretić, Petar ; Musani, Vesna ; Čonkaš, Josipa ; Sabol, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EMBO Workshop Hedgehog signalling: From molecular structure to developmental biology and diseases

Mjesto i datum
Sant Feliu de Guíxols, Španjolska, 24.04.2023. - 27.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hedgehog signaling, GLI targets, HH-GLI

Sažetak
Understanding the interplay of different pathways leading to development and progression of melanoma is important for developing new therapeutic strategies. One of the recent approaches in cancer therapy is targeting the Hedgehog-GLI signaling pathway. To elucidate the role of GLI proteins in melanoma, we performed RNA- sequencing on three human melanoma cell lines with over-expressed GLI1, GLI2 or GLI3. To select GLI target genes for further validation, a list of DEGs was filtered according to FDR values and logFc. After performing KEGG pathway analysis, genes were selected for further investigation according to their role in diseases, role in signaling pathways and their importance in melanoma. 12 genes were chosen for further validation. The validation was performed on 11 additional melanoma cell lines by qPCR. None of the 12 chosen targets are yet reported as GLI targets, and only half of them are recently reported to have a role in melanoma pathogenesis. Also, we found that majority of the genes are overlapping targets of GLI1 and GLI2. Our findings provide new potential targets to consider while designing melanoma-targeted therapy, especially in the case of recurrent disease due to therapy resistance.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA