Naslov
Impact of the zinc complexation of polytopic polyaza ligands on the interaction with double and single stranded DNA/RNA and antimicrobial activity

Autori
González-García, Jorge ; Galiana, Carolina ; Dea-Ayuela, M. Auxiliadora ; Radić Stojković, Marijana ; López-Molina, Sonia ; Galiana-Roselló, Cristina ; Blasco, Salvador ; Piantanida, Ivo ; García-España, Enrique

Izvornik
Dalton transactions (1477-9226) 52 (2023); 5478-5485

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
polyaza ligand ; DNA binding ; RNA binding ; Zinc binding ; antimicrobial activity

Sažetak
Metal complexes have gained a huge interest in the biomedical research in the last decade because of the access to unexplored chemical space with regards to organic molecules and to present additional func-tionalities to act simultaneously as diagnostic and therapeutic agents. Herein, we evaluated the interaction of two polytopic polyaza ligands and their zinc complexes with DNA and RNA by UV thermal denatura-tion, fluorescence and circular dichroism spectroscopic assays. The zinc coordination was investigated by X-ray diffraction and afforded the structure of the binuclear zinc complex of PYPOD. Thermal denaturation of DNA and RNA and fluorimetry analysis revealed preferential binding of the zinc-PHENPOD complexes towards GC-containing DNA in contrast to the free ligands. On the other hand, PYPOD metal complexes, compared to the free ligand, stabilized AT-based DNA (B-form) better than AU-RNA (A-form). With regards to single stranded RNA, the binuclear complex of PHENPOD and the free ligand can efficiently identify polyadenylic acid (poly A) among other RNA sequences by circular dichroism spectroscopy. The antimicrobial activity in S. aureus and E. coli bacteria showed the highest activity for the free ligands and their trinuclear zinc complexes. This work can provide valuable insights into the impact of the nucle-arity of polytopic polyaza ligands in the binding to DNA/RNA and the antimicrobial effect.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA