Histological Type, Cytotoxic T Cells and Macrophages in the Tumor Microenvironment Affect the PD-L1 Status of Gastric Cancer

Ivanović, Tomislav; Božić, Dorotea; Benzon, Benjamin; Čapkun, Vesna; Vukojević, Katarina; Glavina Durdov, Merica

doi:10.3390/biomedicines11030709

Pregled bibliografske jedinice broj: 1268164

Histological Type, Cytotoxic T Cells and Macrophages in the Tumor Microenvironment Affect the PD-L1 Status of Gastric Cancer

Ivanović, Tomislav; Božić, Dorotea; Benzon, Benjamin; Čapkun, Vesna; Vukojević, Katarina; Glavina Durdov, Merica

Histological Type, Cytotoxic T Cells and Macrophages in the Tumor Microenvironment Affect the PD-L1 Status of Gastric Cancer // Biomedicines, 11 (2023), 3; 709, 17 doi:10.3390/biomedicines11030709 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1268164 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Histological Type, Cytotoxic T Cells and Macrophages in the Tumor Microenvironment Affect the PD-L1 Status of Gastric Cancer

Autori
Ivanović, Tomislav ; Božić, Dorotea ; Benzon, Benjamin ; Čapkun, Vesna ; Vukojević, Katarina ; Glavina Durdov, Merica

Izvornik
Biomedicines (2227-9059) 11 (2023), 3; 709, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
rak želudca, imunološki mikrookliš tumora, PD-L1, CTLA-4, INF y
(gastric cancer ; tumor immune microenvironment ; PD-L1 ; CTLA-4 ; IFN-γ)

Sažetak
Gastric cancer (GC) therapies include gastrectomy and chemoradiotherapy. The tumor immune microenvironment (TME) has implications for potential immunotherapy. We analyzed the expression of PD-L1, CD8, CTLA-4 and IFN-γ in the tumor and regional lymph node (LN) of patients with GC and compared it with clinical and pathological data. Paraffin blocks were collected from 97 patients undergoing gastrectomy/lymphadenectomy for GC. Double immunohistochemistry was performed for CD8 and PD- L1 and double immunofluorescence for CTLA-4 and IFN-γ. Statistical significance was set at p < 0.05. PD-L1 expression in tumor cells was associated with intestinal GC type (p = 0.046), the density of macrophages and CD8 + T cells (p < 0.001, both). The median number of CD8+ T cells was higher in PD-L1-positive than in -negative tumors. A cut-off of 28.5 CD8 + T cells in one high-magnification field predicted PD-L1-positive tumors (AUROC 0.797, sensitivity 74.2%, specificity 77.3%). IFN-γ expression in tumor cells was found in 37 GCs and was positively associated with CTLA4+ lymphocytes in the LN (p = 0.027) and CTLA4+/IFN-γ+ in tumors and the LN (all p < 0.001). The median overall survival (OS) was 17 months. In the group of deceased patients, IFN- γ expression in metastases correlated with lower OS (RHO = −0.314, p = 0.008). PD-L1 expression in tumor cells correlated with CD8 + T cells and macrophages in the TME and IFN-γ expression with suppressive CTLA4+/IFNγ+ immune cells in the TME and LN.

Izvorni jezik
Engleski

POVEZANOST RADA

Profili:

Katarina Vukojević (autor)